Yu Shuan Bao, Li Wen Ge, Liu Xiao Shu, Che Jie, Lu Jin Xing, Wu Yuan
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Mycopathologia. 2017 Jun;182(5-6):459-469. doi: 10.1007/s11046-017-0111-2. Epub 2017 Jan 13.
Adhesion and biofilm formation, which can occur on abiotic and biotic surfaces, are key components in Candida pathogenicity. The aims of this study were to infer about the C. tropicalis clinical isolates ability to adhere and form biofilm on abiotic and biotic surfaces and to correlate that with the multilocus sequence typing and other virulence factors. Adhesion and biofilm formation were measured in 68 C. tropicalis isolates from 3 hospitals in China on abiotic (polystyrene) and biotic (human urinary bladder epithelial cell) surfaces by crystal violet assay and 2,3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide reduction assay. In our study, almost all C. tropicalis isolates could adhere and produce biofilm on abiotic and biotic surfaces in a strain-dependent manner. The isolates from blood showed relatively lower adhesion and biofilm capacity on polystyrene surface, but had strong secreted aspartyl proteinase activity. Moreover, significant differences were found among MLST groups for adhesion and biofilm capacity. C. tropicalis in multilocus sequence typing group5 and group6 showed high adhesion and biofilm, while isolates in group1 exhibited low adhesion and biofilm formation. Overall, it is important to note that C. tropicalis isolates adhere to and produce biofilm on abiotic and biotic surfaces with strain specificity. These data will play an important role in subsequent research on the pathogenesis of C. tropicalis.
黏附与生物膜形成可发生在非生物和生物表面，是白色念珠菌致病性的关键组成部分。本研究的目的是推断热带念珠菌临床分离株在非生物和生物表面上黏附及形成生物膜的能力，并将其与多位点序列分型及其他毒力因子相关联。通过结晶紫测定法和2,3-双(2-甲氧基-4-硝基-5-磺基苯基)-2H-四唑-5-甲酰苯胺还原测定法，对来自中国3家医院的68株热带念珠菌分离株在非生物（聚苯乙烯）和生物（人膀胱上皮细胞）表面的黏附与生物膜形成情况进行了测定。在我们的研究中，几乎所有热带念珠菌分离株都能以菌株依赖性方式在非生物和生物表面黏附并产生生物膜。血液来源的分离株在聚苯乙烯表面的黏附及生物膜形成能力相对较低，但具有较强的分泌天冬氨酸蛋白酶活性。此外，多位点序列分型组之间在黏附及生物膜形成能力方面存在显著差异。多位点序列分型第5组和第6组的热带念珠菌表现出高黏附性和生物膜形成能力，而第1组的分离株黏附性和生物膜形成能力较低。总体而言，重要的是要注意到热带念珠菌分离株在非生物和生物表面的黏附及生物膜形成具有菌株特异性。这些数据将在后续热带念珠菌致病机制的研究中发挥重要作用。
