Su Yinxia, Wang Zhiqiang, Yao Hua, Wang Tingting, Ma Qi, Zhu Jun, Wang Shuxia, Ma Yan
Key Laboratory of Metabolic Diease, the First Affiliated Hospital.
Department of Public Health, Xinjiang Medical University, Urumqi 830011, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2015 Oct;36(10):1167-71.
To explore the relationship between the polymorphism of solute carrier family 30, member 8 (SLC30A8) gene and type 2 diabetes mellitus (T2DM) in Uyhgur in Xinjiang and further analyze the interaction between SLC30A8 gene polymorphism loci and smoking.
A case control study, including 1 000 patients with T2DM and 1 010 non-diabetic controls, was conducted in Xinjiang. All the subjects were Uygur and the age difference between the two groups was within 3 years. Physical examination and blood biochemical detection were performed to obtain personal clinical parameters. Genomic DNA was extracted from peripheral blood leukocytes. The single nucleotide polymorphism (SNP) of SLC30A8 of all the subjects was tested by using MALDI-TOF. Statistical analyses were performed with SPSS 16.0. Bootstrap method was used to calculate 95% confidence intervals of RERI, AP and S.
After adjusting BMI, SBP, TC, HDL-C and LDL-C, rs13266634 of SLC30A8 gene genotype frequency and allele frequency distribution had statistical differences (P<0.05). Rs13266634 of risk allele were C, OR was 1.194 (95% CI: 1.044-1.366). In addition, the data from genotype distribution analysis under different models showed that significant association between rs13266634 and T2DM in dominant model, OR was 1.640 (95% CI 1.072-2.510). The product of rs13266634 with the active smoking or passive smoking had no statistical significance (P>0.05) , indicating there were no multiplication interaction among them. Additive interactions index of RERI, AP and S and its 95% confidence interval of rs13266634 and active smoking, rs13266634 and passive smoking were 0.301 (-1.314-0.712), 0.204 (-0.854-0.446), 0.612 (0.186-2.013) and 0.125 (-0.805-1.055), 0.052 (-0.353-0.456), 1.096 (0.500-2.403) respectively, indicating there were no significant additive interaction among them.
Rs13666334 of SLC30A8 gene is associated with the susceptibility of T2DM in Uygur, and its protective genotype might be TT. Passive smoking might increase the risk of T2DM in Uygur.
探讨新疆维吾尔族人群溶质载体家族30成员8（SLC30A8）基因多态性与2型糖尿病（T2DM）的关系，并进一步分析SLC30A8基因多态性位点与吸烟的交互作用。
在新疆进行病例对照研究，纳入1000例T2DM患者和1010例非糖尿病对照者。所有研究对象均为维吾尔族，两组年龄相差3岁以内。进行体格检查和血液生化检测以获取个人临床参数。从外周血白细胞中提取基因组DNA。采用基质辅助激光解析电离飞行时间质谱（MALDI-TOF）检测所有研究对象SLC30A8的单核苷酸多态性（SNP）。应用SPSS 16.0软件进行统计分析。采用Bootstrap法计算相对超额危险度（RERI）、归因比例（AP）和协同指数（S）的95%置信区间。
校正体质指数（BMI）、收缩压（SBP）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）后，SLC30A8基因rs13266634位点的基因型频率和等位基因频率分布有统计学差异（P<0.05）。rs13266634位点的风险等位基因为C，比值比（OR）为1.194（95%置信区间：1.0441.366）。此外，不同模型下的基因型分布分析数据显示，rs13266634位点在显性模型中与T2DM显著相关，OR为1.640（95%置信区间1.0722.510）。rs13266634位点与主动吸烟或被动吸烟的交互作用无统计学意义（P>0.05），提示二者之间不存在相乘交互作用。rs13266634位点与主动吸烟、rs13266634位点与被动吸烟的RERI、AP和S的相加交互作用指数及其95%置信区间分别为0.301（-1.3140.712）、0.204（-0.8540.446）、0.612（0.1862.013）和0.125（-0.8051.055）、0.052（-0.3530.456）、1.096（0.5002.403），提示二者之间不存在显著相加交互作用。
SLC30A8基因rs13666334位点与维吾尔族T2DM易感性相关，其保护性基因型可能为TT。被动吸烟可能增加维吾尔族T2DM发病风险。
