Farmani Fatemeh, Moein Mahmoodreza, Amanzadeh Amir, Kandelous Hirsa Mostafapour, Ehsanpour Zahra, Salimi Mona
Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Tehran, Iran E-mail :
Asian Pac J Cancer Prev. 2016;17(1):315-21. doi: 10.7314/apjcp.2016.17.1.315.
Herbal medicine has becoming a potential source of treatment for different types of cancer including breast cancer. It has been shown that plants from the family Rhamnaceae possess anticancer activity.
In this study, we determined the antiproliferative influence of Ziziphus spina christi- a species from this family- on the MCF-7 (human breast adenocarcinoma) cell line.
The cytotoxicity of the total extract, ethanol, ethanol-aqueous (1:1) as well as aqueous fractions of Ziziphus spina christi leaves was evaluated through MTT assay against MCF-7 cell line. Cell cycle inhibition and apoptosis induction were assessed by flowcytometry cycle RNase/PI analysis and Annexin V-FLUOS, respectively. Apoptosis was also analyzed by immunoblotting assay.
Our results indicated that the ethanolic fraction had the lowest IC50 value (0.02 mg/ml), induced cell cycle arrest at the G1/S phase as well as apoptosis after a 48h of treatment.
This is the first report on anticancer effect of Ziziphus spina christi ethanolic fraction on breast cancer cells, providing a scientific basis for its utility in traditional medicine. However, further in-depth studies are needed to confirm the precise mechanisms.
草药已成为包括乳腺癌在内的不同类型癌症的潜在治疗来源。已表明鼠李科植物具有抗癌活性。
在本研究中，我们测定了该科植物枣（Ziziphus spina christi）对MCF-7（人乳腺腺癌）细胞系的抗增殖影响。
通过MTT法评估枣叶总提取物、乙醇提取物、乙醇 - 水（1:1）提取物以及水提取物对MCF-7细胞系的细胞毒性。分别通过流式细胞术周期RNase/PI分析和膜联蛋白V - FLUOS评估细胞周期抑制和凋亡诱导。还通过免疫印迹分析对凋亡进行了分析。
我们的结果表明，乙醇提取物的IC50值最低（0.02 mg/ml），在处理48小时后诱导细胞周期停滞于G1/S期并诱导凋亡。
这是关于枣乙醇提取物对乳腺癌细胞抗癌作用的首次报道，为其在传统医学中的应用提供了科学依据。然而，需要进一步深入研究以确认确切机制。
