Zuhrotun Nisa Fatma, Astuti Mary, Murdiati Agnes, Mubarika Haryana Sofia
Department of Health and Nutrition, Faculty of Medicine, Universitas Gadjah Mada, Jl Farmako Sekip Utara, 55281 Yogyakarta, Indonesia.
Faculty of Agriculture Technology, Universitas Gadjah Mada, Jl Flora No. 1, 55281 Yogyakarta, Indonesia.
Pak J Biol Sci. 2017;20(1):36-41. doi: 10.3923/pjbs.2017.36.41.
Breast cancer is the most frequently diagnosed cancer in women. Chemotherapy is the main method of breast cancer treatment but there are side effects. Carica papaya leaves is vegetable foods consumed by most people of Indonesia have potential as anticancer. The aim of this study was to investigate anti-proliferative and apoptotic induced effect of aqueous papaya leaves extracts on human breast cancer cell lines MCF-7.
Inhibitory on cell proliferation was measured by MTT assay while apoptosis induction was measured using Annexin V.
The results showed that papaya leaf can inhibit the proliferation of human breast cancer cells MCF-7 with IC50 in 1319.25 μg mL-1. The IC50 values of papaya leaf extract was higher than the IC50 value quercetin and doxorubicin. Papaya leaf extract can also induce apoptosis of breast cancer cells MCF-7 about 22.54% for concentration 659.63 μg mL-1 and about 20.73% for concentration 329.81 μg mL-1. The percentage of cell apoptosis of papaya leaf extract lower than doxorubicin but higher than quercetin.
This study indicated that papaya leaf extract have potential as anticancer through mechanism anti-proliferation and apoptosis induction.
乳腺癌是女性中最常被诊断出的癌症。化疗是乳腺癌治疗的主要方法，但存在副作用。番木瓜叶是印度尼西亚大多数人食用的蔬菜类食物，具有抗癌潜力。本研究的目的是探讨番木瓜叶水提取物对人乳腺癌细胞系MCF-7的抗增殖和诱导凋亡作用。
通过MTT法测定对细胞增殖的抑制作用，使用膜联蛋白V测定凋亡诱导情况。
结果表明，番木瓜叶可抑制人乳腺癌细胞MCF-7的增殖，IC50为1319.25μg/mL。番木瓜叶提取物的IC50值高于槲皮素和阿霉素的IC50值。番木瓜叶提取物还可诱导乳腺癌细胞MCF-7凋亡，浓度为659.63μg/mL时凋亡率约为22.54%，浓度为329.81μg/mL时凋亡率约为20.73%。番木瓜叶提取物的细胞凋亡百分比低于阿霉素但高于槲皮素。
本研究表明，番木瓜叶提取物具有通过抗增殖和诱导凋亡机制发挥抗癌的潜力。
