Williams Tim L, Archer Joy
Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Vet Clin Pathol. 2016 Mar;45(1):148-53. doi: 10.1111/vcp.12324. Epub 2016 Feb 3.
Hyperthyroidism is common in older cats, which necessitates frequent screening of serum total thyroxine (TT4) concentrations. Fast, cheap, and reliable methods to measure TT4 in cats are needed.
The purpose of the study was the validation of a human TT4 enzyme immunoassay (EIA) for use with feline serum, and derivation of a TT4 reference interval (RI) for cats aged 9 years and older.
Assay precision, reproducibility, and linearity were evaluated. Interference by hemolysis was also assessed. Method comparison studies between the human EIA and a previously validated radioimmunoassay (RIA) and chemiluminescent-enzyme immunoassay (CEIA) were performed. Healthy cats (> 9 years) were recruited from 3 UK first opinion practices.
The human TT4 EIA demonstrated good precision and reproducibility, and adequate linearity. Hemolysis did not significantly alter measured TT4 concentrations until HGB > 8 g/L. Method comparison revealed proportional and constant errors between EIA and RIA/CEIA. The TT4 RI for cats (> 9 years) was calculated as 7.1-45.1 nmol/L (n = 49).
The human TT4 EIA was successfully validated for use with feline serum and offers a rapid, cheap, and reliable method for determination of serum TT4 concentrations in cats.
甲状腺功能亢进在老年猫中很常见，这就需要频繁筛查血清总甲状腺素（TT4）浓度。需要快速、廉价且可靠的方法来测量猫的TT4。
本研究的目的是验证一种用于猫血清的人TT4酶免疫测定法（EIA），并推导9岁及以上猫的TT4参考区间（RI）。
评估了测定的精密度、重现性和线性。还评估了溶血的干扰。进行了人EIA与先前验证的放射免疫测定法（RIA）和化学发光酶免疫测定法（CEIA）之间的方法比较研究。从英国3家一级诊疗机构招募了健康猫（>9岁）。
人TT4 EIA显示出良好的精密度和重现性以及足够的线性。直到血红蛋白>8 g/L时，溶血才会显著改变测得的TT4浓度。方法比较显示EIA与RIA/CEIA之间存在比例误差和恒定误差。计算出猫（>9岁）的TT4 RI为7.1 - 45.1 nmol/L（n = 49）。
人TT4 EIA已成功验证可用于猫血清，并为测定猫血清TT4浓度提供了一种快速、廉价且可靠的方法。
