Cox S W, Eley B M
Department of Periodontology, King's College School of Medicine and Dentistry, London, England.
Arch Oral Biol. 1989;34(3):219-21. doi: 10.1016/0003-9969(89)90011-3.
Inflamed gingiva contain a serine proteinase which could not previously be identified on the basis of its substrate specificity and inhibitor response. Using the substrate ZAlaArgArgAFC at alkaline pH, the enzyme was shown to be extracted more efficiently in high salt buffer. Inclusion of NaCl in assays, however, caused progressive reduction of activity. There was also inhibition by CaCl2, MgCl2 and 2 mM TosLysCH2Cl but not by 2 mM TosPheCH2Cl. Heparin produced significant activation. In gel filtrations with 1.0 M NaCl, activity appeared in fractions corresponding to a molecular weight of about 135,000. These properties are all consistent with tryptase from human mast cells. The enzyme may participate in both the connective tissue destruction and the inflammatory and immunological processes of gingivitis and periodontitis.
炎症牙龈中含有一种丝氨酸蛋白酶，以前无法根据其底物特异性和抑制剂反应来鉴定它。在碱性pH条件下使用底物ZAlaArgArgAFC时，该酶在高盐缓冲液中提取效率更高。然而，在测定中加入NaCl会导致活性逐渐降低。CaCl2、MgCl2和2 mM TosLysCH2Cl也有抑制作用，但2 mM TosPheCH2Cl没有。肝素产生显著激活作用。在含1.0 M NaCl的凝胶过滤中，活性出现在对应于分子量约为135,000的级分中。这些特性都与来自人肥大细胞的类胰蛋白酶一致。该酶可能参与了牙龈炎和牙周炎的结缔组织破坏以及炎症和免疫过程。
