Wang Wen, Cao Jian, Yang Fang, Wang Xuelian, Zheng Sisi, Sharshov Kirill, Li Laixing
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xi'ning, 810000, China.
Center of Growth, Metabolism and Aging, College of Life Sciences, Sichuan University, Chengdu, 610000, China.
Microbiologyopen. 2016 Apr;5(2):287-95. doi: 10.1002/mbo3.327. Epub 2016 Feb 4.
Elucidating the spatial dynamic and core gut microbiome related to wild bar-headed goose is of crucial importance for probiotics development that may meet the demands of bar-headed goose artificial breeding industries and accelerate the domestication of this species. However, the core microbial communities in the wild bar-headed geese remain totally unknown. Here, for the first time, we present a comprehensive survey of bar-headed geese gut microbial communities by Illumina high-throughput sequencing technology using nine individuals from three distinct wintering locations in Tibet. A total of 236,676 sequences were analyzed, and 607 OTUs were identified. We show that the gut microbial communities of bar-headed geese have representatives of 14 phyla and are dominated by Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. The additive abundance of these four most dominant phyla was above 96% across all the samples. At the genus level, the sequences represented 150 genera. A set of 19 genera were present in all samples and considered as core gut microbiome. The top seven most abundant core genera were distributed in that four dominant phyla. Among them, four genera (Lactococcus, Bacillus, Solibacillus, and Streptococcus) belonged to Firmicutes, while for other three phyla, each containing one genus, such as Proteobacteria (genus Pseudomonas), Actinobacteria (genus Arthrobacter), and Bacteroidetes (genus Bacteroides). This broad survey represents the most in-depth assessment, to date, of the gut microbes that associated with bar-headed geese. These data create a baseline for future bar-headed goose microbiology research, and make an original contribution to probiotics development for bar-headed goose artificial breeding industries.
阐明与野生斑头雁相关的空间动态和核心肠道微生物群，对于开发满足斑头雁人工养殖产业需求并加速该物种驯化的益生菌至关重要。然而，野生斑头雁的核心微生物群落仍然完全未知。在此，我们首次使用来自西藏三个不同越冬地点的九只个体，通过Illumina高通量测序技术对斑头雁肠道微生物群落进行了全面调查。共分析了236,676个序列，鉴定出607个操作分类单元（OTU）。我们发现斑头雁的肠道微生物群落有14个门的代表，主要由厚壁菌门、变形菌门、放线菌门和拟杆菌门主导。在所有样本中，这四个最主要门的累加丰度超过96%。在属水平上，这些序列代表了150个属。一组19个属存在于所有样本中，被认为是核心肠道微生物群。最丰富的前七个核心属分布在那四个优势门中。其中，四个属（乳球菌属、芽孢杆菌属、固氮芽孢杆菌属和链球菌属）属于厚壁菌门，而其他三个门各包含一个属，如变形菌门（假单胞菌属）、放线菌门（节杆菌属）和拟杆菌门（拟杆菌属）。这项广泛的调查是迄今为止对与斑头雁相关的肠道微生物进行的最深入评估。这些数据为未来斑头雁微生物学研究奠定了基础，并为斑头雁人工养殖产业的益生菌开发做出了原创性贡献。
