State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, Qinghai Province, China.
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, Qinghai Province, China.
Arch Microbiol. 2020 Jul;202(5):983-993. doi: 10.1007/s00203-019-01802-0. Epub 2020 Jan 4.
The black-necked crane (Grus nigricollis) is a vulnerable species, breeding exclusively on the high-altitude wetlands of the Qinghai-Tibet Plateau. Bird species harbor diverse communities of microorganisms within their gastrointestinal tracts, which have important roles in the health, nutrition, and physiology of birds. Hitherto, virtually nothing was known about the gut microbial communities associated with wild black-necked cranes. For the first time, this study characterized the gut microbial community compositions, diversity, and functions of black-necked cranes from six wintering areas in China using the Illumina Miseq platform. The taxonomic results revealed that Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the four most abundant phyla in the gut of black-necked cranes. At the genus level, 11 genera including Lactobacillus, Pseudomonas, Carnobacterium, Pantoea, Enterococcus, Erwinia, Turicibacter, Bacillus, Phenylobacterium, Sanguibacter, and Psychrobacter were dominant. The differences in the gut microbial community alpha and the beta diversities of black-necked cranes among the six wintering areas were investigated. Furthermore, the representative microbial taxa and their predicted functions in each wintering location were also determined. These data represent the first analysis of the gut microbiome of black-necked cranes, providing a baseline for further microbiological studies and a foundation for the conservation of this bird.
黑颈鹤（Grus nigricollis）是一种易危物种，仅在青藏高原的高海拔湿地繁殖。鸟类的胃肠道内栖息着多种多样的微生物群落，这些微生物在鸟类的健康、营养和生理机能方面发挥着重要作用。迄今为止，人们对野生黑颈鹤的胃肠道微生物群落几乎一无所知。本研究首次利用 Illumina Miseq 平台，对来自中国六个越冬地的黑颈鹤的胃肠道微生物群落组成、多样性和功能进行了研究。分类学结果表明，厚壁菌门、变形菌门、放线菌门和拟杆菌门是黑颈鹤胃肠道中最丰富的四个菌门。在属水平上，11 个属包括乳杆菌属、假单胞菌属、肉杆菌属、泛菌属、肠球菌属、欧文氏菌属、图里西杆菌属、芽孢杆菌属、苯基杆菌属、 sanguibacter 属和 Psychrobacter 属，占主导地位。研究还调查了黑颈鹤在六个越冬地的胃肠道微生物群落 α 和 β 多样性的差异。此外，还确定了每个越冬地代表性的微生物类群及其预测功能。这些数据代表了对黑颈鹤肠道微生物组的首次分析，为进一步的微生物学研究提供了基线，并为保护这种鸟类提供了基础。
