Yamamoto-Hino Miki, Goto Satoshi
Department of Life Science, Rikkyo University.
Cell Struct Funct. 2016 May 7;41(1):55-60. doi: 10.1247/csf.16002. Epub 2016 Feb 4.
The Toll pathway regulates innate immunity in insects and vertebrates. The Drosophila Toll receptor is activated by a processed form of a ligand, Spätzle. Spätzle-processing enzyme (SPE) is the only enzyme identified to date that functions in converting Spätzle to an active form during the immune response. In the present study, Toll activation induced by immune challenge was almost suppressed in spätzle mutant larvae and adults, whereas it was present in SPE mutant larvae challenged with Micrococcus luteus and adults challenged with Bacillus subtilis. Our data suggest that an unidentified protease besides SPE processes Spätzle under conditions of microbial challenge.
Toll信号通路调节昆虫和脊椎动物的先天免疫。果蝇的Toll受体由配体Spätzle的一种加工形式激活。Spätzle加工酶（SPE）是迄今为止鉴定出的唯一一种在免疫反应期间将Spätzle转化为活性形式的酶。在本研究中，免疫攻击诱导的Toll激活在spätzle突变体幼虫和成虫中几乎受到抑制，而在用藤黄微球菌攻击的SPE突变体幼虫和用枯草芽孢杆菌攻击的成虫中则存在Toll激活。我们的数据表明，除了SPE之外，一种未鉴定的蛋白酶在微生物攻击条件下加工Spätzle。
