Saxena Hari Mohan, Chothe Shubhada, Kaur Paviter
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev, Veterinary and Animal Sciences University (GADVASU), Ludhiana 141004, India.
MethodsX. 2015 Sep 5;2:345-52. doi: 10.1016/j.mex.2015.08.001. eCollection 2015.
We have developed a new Superagglutination test for serodiagnosis of infectious diseases. It differs from conventional plate/slide agglutination tests (PAT/SAT) by three additional steps: prior staining of serum antibody by adding a dye and addition of diluted biotinylated antiglobulin and avidin in sequence after mixing the antigen with the test serum. The new steps circumvent the problems of false positive and false negative results of PAT/SAT. In serodiagnosis of brucellosis, Superagglutination test had higher positive predictive value and specificity than Rose Bengal Plate Test (RBPT) and Standard Tube Agglutination Test (STAT) and higher negative predictive value and sensitivity than RBPT, STAT, ELISA and Complement Fixation Test (CFT).•Superagglutination is a simple, accurate and economic screening test for infections.•More specificity, sensitivity, positive & negative predictive value than RBPT, STAT.•More sensitivity, negative predictive value than ELISA and Complement Fixation Test.
我们开发了一种用于传染病血清诊断的新型超级凝集试验。它与传统的平板/玻片凝集试验（PAT/SAT）不同，增加了三个步骤：通过添加染料对血清抗体进行预先染色，以及在将抗原与测试血清混合后依次添加稀释的生物素化抗球蛋白和抗生物素蛋白。这些新步骤规避了PAT/SAT出现假阳性和假阴性结果的问题。在布鲁氏菌病的血清诊断中，超级凝集试验比玫瑰红平板试验（RBPT）和标准试管凝集试验（STAT）具有更高的阳性预测值和特异性，并且比RBPT、STAT、酶联免疫吸附测定（ELISA）和补体结合试验（CFT）具有更高的阴性预测值和敏感性。
•超级凝集试验是一种用于感染的简单、准确且经济的筛查试验。
•比RBPT、STAT具有更高的特异性、敏感性、阳性和阴性预测值。
•比ELISA和补体结合试验具有更高的敏感性和阴性预测值。
