Fukuda Hiroki, Nakamura Seikou, Chisaki Yugo, Takada Tetsuya, Toda Yuki, Murata Hiroaki, Itoh Kazuyuki, Yano Yoshitaka, Takata Kazuyuki, Ashihara Eishi
Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Kyoto, Japan.
Department of Pharmacognosy, Kyoto Pharmaceutical University, Kyoto, Japan.
Biochem Biophys Res Commun. 2016 Feb 26;471(1):63-7. doi: 10.1016/j.bbrc.2016.01.179. Epub 2016 Feb 1.
Daphnetin, 7,8-dihydroxycoumarin, present in main constituents of Daphne odora var. marginatai, has multiple pharmacological activities including anti-proliferative effects in cancer cells. In this study, using a Transwell system, we showed that daphnetin inhibited invasion and migration of highly metastatic murine osteosarcoma LM8 cells in a dose-dependent manner. Following treatment by daphnetin, cells that penetrated the Transwell membrane were rounder than non-treated cells. Immunofluorescence analysis revealed that daphnetin decreased the numbers of intracellular stress fibers and filopodia. Moreover, daphnetin treatment dramatically decreased the expression levels of RhoA and Cdc42. In summary, the dihydroxycoumarin derivative daphnetin inhibits the invasion and migration of LM8 cells, and therefore represents a promising agent for use against metastatic cancer.
瑞香素，即7,8 - 二羟基香豆素，是金边瑞香主要成分之一，具有多种药理活性，包括对癌细胞的抗增殖作用。在本研究中，我们使用Transwell系统表明，瑞香素以剂量依赖性方式抑制高转移性小鼠骨肉瘤LM8细胞的侵袭和迁移。用瑞香素处理后，穿透Transwell膜的细胞比未处理的细胞更圆。免疫荧光分析显示，瑞香素减少了细胞内应力纤维和丝状伪足的数量。此外，瑞香素处理显著降低了RhoA和Cdc42的表达水平。总之，二羟基香豆素衍生物瑞香素抑制LM8细胞的侵袭和迁移，因此是一种有前景的抗转移性癌症药物。
