Jeswin Joseph, Xie Xiao-lu, Ji Qiao-lin, Wang Ke-jian, Liu Hai-peng
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, Fujian, PR China.
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, Fujian, PR China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian Engineering Laboratory of Marine Bioproducts and Technology, Xiamen 361102, Fujian, PR China.
Fish Shellfish Immunol. 2016 Mar;50:288-96. doi: 10.1016/j.fsi.2016.01.035. Epub 2016 Feb 1.
To elucidate proteomic changes of Hpt cells from red claw crayfish, Cherax quadricarinatus, we have carried out isobaric tags for relative and absolute quantitation (iTRAQ) of cellular proteins at both early (1 hpi) and late stage (12 hpi) post white spot syndrome virus (WSSV) infection. Protein database search revealed 594 protein hits by Mascot, in which 17 and 30 proteins were present as differentially expressed proteins at early and late viral infection, respectively. Generally, these differentially expressed proteins include: 1) the metabolic process related proteins in glycolysis and glucogenesis, DNA replication, nucleotide/amino acid/fatty acid metabolism and protein biosynthesis; 2) the signal transduction related proteins like small GTPases, G-protein-alpha stimulatory subunit, proteins bearing PDZ- or 14-3-3-domains that help holding together and organize signaling complexes, casein kinase I and proteins of the MAP-kinase signal transduction pathway; 3) the immune defense related proteins such as α-2 macroglobulin, transglutaminase and trans-activation response RNA-binding protein 1. Taken together, these protein information shed new light on the host cellular response against WSSV infection in a crustacean cell culture.
为阐明红螯螯虾（Cherax quadricarinatus）血细胞（Hpt细胞）的蛋白质组变化，我们对白斑综合征病毒（WSSV）感染早期（感染后1小时）和晚期（感染后12小时）的细胞蛋白质进行了相对和绝对定量的等压标签标记（iTRAQ）。蛋白质数据库搜索通过Mascot软件鉴定出594个蛋白质匹配结果，其中分别有17个和30个蛋白质在病毒感染早期和晚期呈现差异表达。总体而言，这些差异表达的蛋白质包括：1）糖酵解和糖异生、DNA复制、核苷酸/氨基酸/脂肪酸代谢以及蛋白质生物合成等代谢过程相关蛋白；2）信号转导相关蛋白，如小GTP酶、G蛋白α刺激亚基、带有PDZ或14-3-3结构域以帮助信号复合物聚集和组织的蛋白、酪蛋白激酶I以及丝裂原活化蛋白激酶信号转导途径的蛋白；3）免疫防御相关蛋白，如α-2巨球蛋白、转谷氨酰胺酶和反式激活应答RNA结合蛋白1。综上所述，这些蛋白质信息为甲壳类细胞培养中宿主细胞对WSSV感染的应答提供了新的见解。
