State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen, 361102, Fujian, PR China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen, 361102, Fujian, PR China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, PR China.
Dev Comp Immunol. 2020 Oct;111:103749. doi: 10.1016/j.dci.2020.103749. Epub 2020 Jun 4.
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is pivotal in immune responses for a variety of pathogens in both vertebrates and invertebrates. Domeless (Dome), as a unique cytokine receptor, involves in the upstream JAK/STAT pathway in invertebrates. In this study, the full-length cDNA sequence of a cytokine receptor Dome was identified from red claw crayfish Cherax quadricarinatus (named as CqDome), which contained an open reading frame of 4251 bp, encoding 1416 amino acids. The CqDome contained extracellular conservative domains of a signal peptide, two cytokine binding modules (CBM), three fibronectin-type-III-like (FN3) domains and a transmembrane region. Tissue distribution analysis showed that CqDome generally expressed in all the tissues selected with a high expression in hemocyte. The gene expression of both the viral immediately early gene (IE1) and a late gene envelope protein VP28 of white spot syndrome virus (WSSV) were significantly decreased after gene silencing of CqDome in crayfish haematopoietic tissue (Hpt) cells, indicating a key role of CqDome in promoting WSSV infection. Furthermore, the phosphorylation level of CqSTAT was significantly inhibited by gene silencing of CqDome in Hpt cells, indicating that CqDome participated in signal transduction of JAK/STAT pathway in red claw crayfish. These data together suggest that CqDome is likely to promote WSSV infection via JAK/STAT pathway, which sheds new light on further elucidation of the pathogenesis of WSSV.
Janus 激酶/信号转导和转录激活因子（JAK/STAT）信号通路在脊椎动物和无脊椎动物的各种病原体的免疫反应中起着至关重要的作用。无顶（Dome）作为一种独特的细胞因子受体，参与无脊椎动物上游的 JAK/STAT 通路。在本研究中，从红螯螯虾 Cherax quadricarinatus 中鉴定出一种细胞因子受体 Dome 的全长 cDNA 序列（命名为 CqDome），其包含 4251 bp 的开放阅读框，编码 1416 个氨基酸。CqDome 包含信号肽的细胞外保守结构域、两个细胞因子结合模块（CBM）、三个纤维连接蛋白 III 样（FN3）结构域和一个跨膜区域。组织分布分析表明，CqDome 在所选的所有组织中均普遍表达，在血细胞中表达水平较高。在螯虾造血组织（Hpt）细胞中沉默 CqDome 基因后，白斑综合征病毒（WSSV）的病毒立即早期基因（IE1）和晚期基因包膜蛋白 VP28 的基因表达均显著降低，表明 CqDome 在促进 WSSV 感染中起关键作用。此外，在 Hpt 细胞中沉默 CqDome 基因后，CqSTAT 的磷酸化水平显著受到抑制，表明 CqDome 参与了红螯螯虾 JAK/STAT 通路的信号转导。这些数据共同表明，CqDome 可能通过 JAK/STAT 通路促进 WSSV 感染，这为进一步阐明 WSSV 的发病机制提供了新的线索。
