用于检测热变性胶原蛋白的球形银纳米颗粒。
Spherical silver nanoparticles in the detection of thermally denatured collagens.
作者信息
Ahumada Manuel, McLaughlin Sarah, Pacioni Natalia L, Alarcon Emilio I
机构信息
Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Rm H5229, Ottawa, Ontario, K1Y 4W7, Canada.
Laboratorio de Cinética y Fotoquímica, Departamento de Ciencias del Ambiente-Facultad de Química y Biología, Universidad de Santiago de Chile, Avenida Libertador Bernardo O´higgins 3363, 8370071, Santiago, Chile.
出版信息
Anal Bioanal Chem. 2016 Mar;408(8):1993-6. doi: 10.1007/s00216-016-9330-5. Epub 2016 Feb 4.
We have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation. Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution of pure collagen was 14.7, 8.5, and 8.6 μg mL(-1) for porcine (PCOL), rat tail (RCOL), and type I human recombinant (HCOL) collagen, respectively.
我们开发了一种快速比色法来测定溶液中变性胶原蛋白的浓度，该方法基于胶原蛋白-银纳米颗粒冠层的形成。使用所提出的方法，对于猪（PCOL）、大鼠尾巴（RCOL）和I型人重组（HCOL）胶原蛋白，纯胶原蛋白溶液中变性胶原蛋白蛋白的最低可检测浓度分别为14.7、8.5和8.6 μg mL(-1)。
Zotero 插件