Wen Xinzhu, Wang Nan, Zhang Fanfan, Dong Chunjiao
Department of Orthopedics, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, P.R. China.
Department of Peripheral Vascular Surgery, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, P.R. China.
Mol Med Rep. 2016 Mar;13(3):2885-91. doi: 10.3892/mmr.2016.4857. Epub 2016 Feb 3.
Scavenger receptor class A, member 5 (SCARA5) is a member of the scavenger receptor family, and is involved in several types of human malignancy; however, its roles in osteosarcoma (OS) remain to be fully elucidated. Therefore, in the present study, the biological functions of SCARA5 in OS, and the potential underlying mechanisms were investigated. SCARA5 expression in OS tissues and cell lines was detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The effects of SCARA5 on the proliferation and migration/invasion ability of OS cells were determined by MTT and Transwell chamber assays, respectively. Expression levels of phosphorylated focal adhesion kinase (p‑FAK), FAK, p‑Src, Src, matrix metalloproteinase (MMP)2 and MMP9 were evaluated via western blot analysis. The results of the present study demonstrated that SCARA5 was expressed at low levels in OS tissues and cell lines. The overexpression of SCARA5 significantly inhibited the proliferation, colony formation and migration/invasion abilities of the OS cells. Furthermore, SCARA5 significantly decreased the expression levels of p‑FAK, MMP‑2 and MMP‑9 in the OS cells. Taken together, these data suggested that the overexpression of SCARA5 inhibits tumor proliferation and invasion in OS via suppression of the FAK signaling pathway. Thus, novel therapeutic strategies or drugs targeted at SCARA5 may offer potential for the treatment of OS.
清道夫受体A类成员5（SCARA5）是清道夫受体家族的一员，参与多种人类恶性肿瘤；然而，其在骨肉瘤（OS）中的作用仍有待充分阐明。因此，在本研究中，对SCARA5在骨肉瘤中的生物学功能及其潜在机制进行了研究。通过逆转录-定量聚合酶链反应和蛋白质印迹分析检测骨肉瘤组织和细胞系中SCARA5的表达。分别通过MTT和Transwell小室试验确定SCARA5对骨肉瘤细胞增殖和迁移/侵袭能力的影响。通过蛋白质印迹分析评估磷酸化粘着斑激酶（p-FAK）、FAK、p-Src、Src、基质金属蛋白酶（MMP）2和MMP9的表达水平。本研究结果表明，SCARA5在骨肉瘤组织和细胞系中低表达。SCARA5的过表达显著抑制了骨肉瘤细胞的增殖、集落形成以及迁移/侵袭能力。此外，SCARA5显著降低了骨肉瘤细胞中p-FAK、MMP-2和MMP-9的表达水平。综上所述，这些数据表明SCARA5的过表达通过抑制FAK信号通路抑制骨肉瘤的肿瘤增殖和侵袭。因此，针对SCARA5的新型治疗策略或药物可能为骨肉瘤的治疗提供潜力。
