Ni Qinggan, Li Xia, Huang Hua, Ge Zili
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Department of Burns and Plastic Surgery, Yancheng Clinical College of Xuzhou Medical University, The First People's Hospital of Yancheng, Yancheng, China.
Front Oncol. 2023 Mar 24;13:1015358. doi: 10.3389/fonc.2023.1015358. eCollection 2023.
It has been established that the scavenger receptor class A member 5 () functions as a tumor suppressor gene in various cancer types. To our knowledge, no comprehensive study has hitherto investigated the expression and function of in melanoma. This study aimed to determine the association between and melanoma.
Analysis of mRNA expression was performed using The Cancer Genome Atlas (TCGA) data sets. To evaluate the clinical significance of , the clinical data of 93 patients with melanoma were collected. The role of expression in prognosis was also analyzed. In this study, survival was evaluated by Kaplan-Meier analysis and compared using the log-rank test. Univariate and multivariate Cox proportional hazard regression analyses were used to identify independent predictors. The Kyoto Encyclopedia of Genes and Genomes, Gene Ontology, and gene set enrichment analysis (GSEA) were used to perform gene set functional annotations. Protein-protein interaction (PPI) networks were constructed to illustrate gene-gene interactions. The Tumor IMmune Estimation Resource (TIMER) database was used to explore the association between and immune infiltration levels.
The results showed that the mRNA expression in melanoma was significantly lower than in adjacent normal skin tissue ( < 0.001). Moreover, decreased expression of in melanoma correlated with the tumor, node, and metastasis (TNM) stage and recurrence ( < 0.05). The overall survival (OS) was significantly higher in melanoma with high expression compared with low expression ( < 0.001). During univariate analysis, expression, tumor (T) stage, node (N) stage, metastasis (M) stage, and recurrence correlated with OS ( < 0.05). Further multivariate Cox regression analysis showed that expression ( = 0.012) could be an independent prognostic factor for OS in cutaneous malignant melanoma. GSEA analysis showed that was significantly enriched in various pathways, such as response to developmental biology and response to antimicrobial peptides. Correlation analysis showed a positive correlation with CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells ( < 0.05), and a negative correlation with tumor purity ( < 0.05).
has significant potential as a prognostic biomarker and as a promising therapeutic target in melanoma. Furthermore, expression in melanoma is related to the level of immune infiltration.
已有研究证实，清道夫受体A类成员5（）在多种癌症类型中发挥肿瘤抑制基因的作用。据我们所知，迄今为止尚无全面研究调查其在黑色素瘤中的表达及功能。本研究旨在确定与黑色素瘤之间的关联。
使用癌症基因组图谱（TCGA）数据集对的mRNA表达进行分析。为评估的临床意义，收集了93例黑色素瘤患者的临床数据。还分析了表达在预后中的作用。在本研究中，通过Kaplan-Meier分析评估生存率，并使用对数秩检验进行比较。采用单因素和多因素Cox比例风险回归分析来确定独立预测因素。利用京都基因与基因组百科全书、基因本体论和基因集富集分析（GSEA）进行基因集功能注释。构建蛋白质-蛋白质相互作用（PPI）网络以阐明基因-基因相互作用。使用肿瘤免疫估计资源（TIMER）数据库探索与免疫浸润水平之间的关联。
结果显示，黑色素瘤中的mRNA表达显著低于相邻正常皮肤组织（<0.001）。此外，黑色素瘤中表达降低与肿瘤、淋巴结和转移（TNM）分期及复发相关（<0.05）。高表达的黑色素瘤患者的总生存期（OS）显著高于低表达患者（<0.001）。在单因素分析中，表达、肿瘤（T）分期、淋巴结（N）分期、转移（M）分期和复发与OS相关（<0.05）。进一步的多因素Cox回归分析表明，表达（=0.012）可能是皮肤恶性黑色素瘤OS的独立预后因素。GSEA分析表明，在多种途径中显著富集，如对发育生物学的反应和对抗菌肽的反应。相关性分析显示与CD8 + T细胞、CD4 + T细胞、巨噬细胞、中性粒细胞和树突状细胞呈正相关（<0.05），与肿瘤纯度呈负相关（<0.05）。
在黑色素瘤中具有作为预后生物标志物和有前景的治疗靶点的显著潜力。此外，黑色素瘤中的表达与免疫浸润水平相关。
