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评价免疫层析试验用于快速检测粪便样本中新出现的 GII.17 诺如病毒,2016 年 1 月。

Evaluation of immunochromatographic tests for the rapid detection of the emerging GII.17 norovirus in stool samples, January 2016.

机构信息

National Reference Center for Enteric Viruses, Laboratory of Virology, University Hospital of Dijon, Dijon, France.

出版信息

Euro Surveill. 2016;21(4). doi: 10.2807/1560-7917.ES.2016.21.4.30115.

Abstract

A novel GII.17 norovirus emerged in Asia in the winter of 2014/15. A worldwide spread is conceivable and norovirus diagnostic assays need to be evaluated to investigate if they adequately detect this emerging genotype. Seven immunochromatographic kits commercially available in Europe were evaluated on ten stool samples where GII.17 virus had been quantified by real-time reverse transcription-polymerase chain reaction. All the kits detected GII.17 with various sensitivities, partly depending on the virus titre.

摘要

一种新型的 GII.17 诺如病毒于 2014/15 年冬季在亚洲出现。可以想象这种病毒会在全球范围内传播,因此需要对诺如病毒诊断检测方法进行评估,以确定它们是否能够充分检测到这种新出现的基因型。对十种粪便样本进行了评估,这些样本中通过实时逆转录-聚合酶链反应定量检测到了 GII.17 病毒,这些样本来自欧洲的十种商业上可获得的免疫胶体金试剂盒。所有试剂盒均以不同的灵敏度检测到了 GII.17,部分灵敏度取决于病毒滴度。

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