免疫层析法检测诺如病毒的效率评估与比较

Evaluation and comparison of the efficiency of immunochromatography methods for norovirus detection.

作者信息

Thongprachum Aksara, Khamrin Pattara, Tran Dinh Nguyen, Okitsu Shoko, Mizuguchi Masashi, Hayakawa Satoshi, Maneekarn Niwat, Ushijima Hiroshi

机构信息

Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

出版信息

Clin Lab. 2012;58(5-6):489-93.

PMID:22783579

Abstract

BACKGROUND

The efficiency of a commercial immunochromatography kit (IP-NoV) was evaluated for rapid detection of norovirus in Japanese fecal specimens and its sensitivity and specificity were compared with two other commercial kits.

METHODS

A total of 70 samples collected from children who suffered from acute gastroenteritis in 2009 - 2010 were tested for norovirus by three different immunochromatography kits. RT-PCR was employed as the gold standard method.

RESULTS

The sensitivity of IP-NoV, QuickEx-Norovirus, and QuickNavi-Norovirus kits were 85.2%, 63.9%, and 55.7%, respectively. The IP-NoV kit could detect various genotypes of norovirus with higher efficiency as compared to QuickEx-Norovirus and QuickNavi-Norovirus kits. In addition, the phylogenetic analysis revealed that the majority of noroviruses circulating in Japan during 2009 - 2010 belonged to GII/4 variant 2006b and 2008a, which were responsible for the outbreaks worldwide.

CONCLUSIONS

The findings indicated that the IC kits could be used as an alternative method for direct detection of norovirus in clinical specimens covering a wide range of norovirus genotypes circulating in Japan.

摘要

背景

评估了一种商用免疫层析试剂盒（IP-NoV）在快速检测日本粪便标本中诺如病毒方面的效率，并将其灵敏度和特异性与其他两种商用试剂盒进行了比较。

方法

使用三种不同的免疫层析试剂盒对2009年至2010年从患有急性胃肠炎的儿童中收集的70份样本进行诺如病毒检测。采用逆转录聚合酶链反应（RT-PCR）作为金标准方法。

结果

IP-NoV、QuickEx-Norovirus和QuickNavi-Norovirus试剂盒的灵敏度分别为85.2%、63.9%和55.7%。与QuickEx-Norovirus和QuickNavi-Norovirus试剂盒相比，IP-NoV试剂盒能够更高效地检测出各种基因型的诺如病毒。此外，系统发育分析表明，2009年至2010年期间在日本传播的大多数诺如病毒属于GII/4变异株2006b和2008a，这些变异株在全球范围内引发了疫情。