Ushijima Hiroshi, Thongprachum Aksara, Khamrin Pattara, Takanashi Sayaka, Okitsu Shoko, Maneekarn Niwat, Hayakawa Satoshi
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo.
Jpn J Infect Dis. 2017 May 24;70(3):326-328. doi: 10.7883/yoken.JJID.2016.413. Epub 2016 Dec 22.
A novel GII.17 norovirus (NoV), Kawasaki 2014, has spread to several regions of the world. Rapid and reliable diagnostic tests are needed for the detection of this new NoV variant. In this study,analytical sensitivity of 7 different immunochromatographic (IC) test kits (6 are on the market in Japan and one in Europe) was evaluated by means of confirmed GII.17 NoV-positive stool samples. The stool samples were also tested by a bioluminescent enzyme immunoassay (BLEIA). Real-time RT-PCR served as a reference (gold standard) method. Among the 7 IC kits, RIDA QUICK was the most sensitive, with the limit of detection of 10 copies/ml, whereas the limits of detection of the other IC kits ranged from 10 to 10 copies/ml. It should be pointed out that the limit of detection of BLEIA was approximately 100- to 1,000-fold better (10-10 copies/ml) than that of RIDA QUICK. Nevertheless, the procedure of BLEIA took more time and required sophisticated equipment.
一种新型的GII.17诺如病毒（NoV），即2014年川崎株，已传播至世界多个地区。检测这种新的诺如病毒变体需要快速可靠的诊断测试。在本研究中，通过已确认的GII.17诺如病毒阳性粪便样本评估了7种不同免疫层析（IC）测试试剂盒（6种在日本市场销售，1种在欧洲市场销售）的分析灵敏度。粪便样本也通过生物发光酶免疫测定法（BLEIA）进行检测。实时逆转录聚合酶链反应（RT-PCR）作为参考（金标准）方法。在这7种IC试剂盒中，RIDA QUICK最为灵敏，检测限为10拷贝/毫升，而其他IC试剂盒的检测限在10至10拷贝/毫升之间。需要指出的是，BLEIA的检测限比RIDA QUICK大约高100至1000倍（10至10拷贝/毫升）。然而，BLEIA的检测过程耗时更长，且需要精密设备。
