Jurczak Anna, Szkup Małgorzata, Grzywacz Anna, Safranow Krzysztof, Grochans Elżbieta
Department of Nursing, Pomeranian Medical University in Szczecin, Żołnierska 48, 71-210 Szczecin, Poland.
Department of Psychiatry, Pomeranian Medical University in Szczecin, Broniewskiego 26, 71-460 Szczecin, Poland.
Int J Environ Res Public Health. 2016 Feb 2;13(2):185. doi: 10.3390/ijerph13020185.
The objective of this work was the analysis of the relationships between the genotypes of the AMH and AMH receptor type 2 genes, hormone levels and the menstrual cycle in a group of Polish women in the late reproductive stage. The study was conducted using a measurement-based method (body weight and height), laboratory method (serum hormone levels AMH, FSH and E2), and genetic analysis (DNA isolated from whole blood by a salting-out method). The study involved 345 healthy, late-reproductive-stage women from Poland, aged 42.3 ± 4.5 years. The analysis demonstrated that neither the T/T and G/T+G/G genotypes of the AMH Ile(49)Ser polymorphism (rs10407022), nor the A/A and the G/A + G/G genotypes of the AMHR2 2482 A > G polymorphism (rs2002555), nor the C/C and C/T + T/T genotypes of the AMH polymorphism (rs11170547) were statistically significantly related (p > 0.05) to such factors as age, BMI, hormone (FSH and E₂) levels and ovarian parameters (AMH) in the follicular phase. No relationships were found between ovarian parameters (FSH, E₂, AMH) and genetic variants of AMH (rs10407022) and AMHR2 (rs11170547, rs2002555) in healthy women in the late reproductive stage.
这项工作的目的是分析一组处于生殖后期的波兰女性中抗缪勒管激素（AMH）和2型AMH受体基因的基因型、激素水平与月经周期之间的关系。该研究采用了基于测量的方法（体重和身高）、实验室方法（血清激素水平AMH、促卵泡生成素（FSH）和雌二醇（E2））以及基因分析（通过盐析法从全血中分离DNA）。该研究纳入了345名来自波兰的健康、处于生殖后期的女性，年龄为42.3±4.5岁。分析表明，AMH Ile(49)Ser多态性（rs10407022）的T/T和G/T + G/G基因型、AMHR2 2482 A>G多态性（rs2002555）的A/A和G/A + G/G基因型以及AMH多态性（rs11170547）的C/C和C/T + T/T基因型，与卵泡期的年龄、体重指数（BMI）、激素（FSH和E₂）水平及卵巢参数（AMH）等因素均无统计学显著相关性（p>0.05）。在处于生殖后期的健康女性中，未发现卵巢参数（FSH、E₂、AMH）与AMH（rs10407022）及AMHR2（rs11170547、rs2002555）的基因变异之间存在关联。
