Swami Shelesh Kumar, Vijay Anushri, Nagarajan Govindasamy, Kaur Ramneek, Srivastava Meera
a Singhania University , Jhunjhunu , Rajasthan , India.
b Southern Regional Research Center , Central Sheep and Wool Research Institute , Mannavanur, Kodaikanal, Dindigul District , Tamil Nadu , India.
Anim Biotechnol. 2016;27(1):66-76. doi: 10.1080/10495398.2015.1088449.
Interleukin (IL)-1β and IL-8 are pro-inflammatory cytokines produced primarily by monocytes and macrophages in response to a variety of microbial and nonmicrobial agents. As yet, no molecular data have been reported for IL-1β and IL-8 of the Asian elephant. In the present study, we have cloned and sequenced the cDNA encoding IL-1β and IL-8 of the Asian elephant. The open reading frame (ORF) of Asian elephant IL-1β is 789 bp in length, encoded a propeptide of 263 amino acid polypeptide. The predicted protein revealed the presence of IL-1 family signature motif and an ICE cut site. Whereas, IL-8 contained 321 bp of open reading frame. Interestingly, the predicted protein sequence of 106 aa, contains an ELR motif immediately upstream of the CQC residues, common in all vertebrate IL-8 molecules. Identity levels of the nucleic acid and deduced amino acid sequences of Asian elephant IL-1β ranged from 68.48 (Squirrel monkey) to 98.57% (African elephant), and 57.78 (Sheep) to 98.47% (African elephant), respectively, whereas that of IL-8 ranged from 72.9% (Human) to 87.8% (African elephant), and 63.2 (human, gorilla, chimpanzee) to 74.5% (African elephant, buffalo), respectively. The phylogenetic analysis based on deduced amino acid sequenced showed that the Asian elephant IL-1β and IL-8 were most closely related to African elephant. Molecular characterization of these two cytokines, IL-1β and IL-8, in Asian elephant provides fundamental information necessary to progress the study of functional immune responses in this animal and gives the potential to use them to manipulate the immune response as recombinant proteins.
白细胞介素（IL）-1β和IL-8是主要由单核细胞和巨噬细胞产生的促炎细胞因子，以响应多种微生物和非微生物因子。迄今为止，尚未有关于亚洲象IL-1β和IL-8的分子数据报道。在本研究中，我们克隆并测序了编码亚洲象IL-1β和IL-8的cDNA。亚洲象IL-1β的开放阅读框（ORF）长度为789 bp，编码一个由263个氨基酸多肽组成的前体肽。预测的蛋白质显示存在IL-1家族特征基序和一个ICE切割位点。而IL-8包含321 bp的开放阅读框。有趣的是，预测的106个氨基酸的蛋白质序列在CQC残基上游紧邻一个ELR基序，这在所有脊椎动物IL-8分子中都很常见。亚洲象IL-1β的核酸和推导氨基酸序列的同一性水平分别为68.48%（松鼠猴）至98.57%（非洲象），以及57.78%（绵羊）至98.47%（非洲象），而IL-8的同一性水平分别为72.9%（人类）至87.8%（非洲象），以及63.2%（人类、大猩猩、黑猩猩）至74.5%（非洲象、水牛）。基于推导氨基酸序列的系统发育分析表明，亚洲象IL-1β和IL-8与非洲象关系最为密切。对亚洲象这两种细胞因子IL-1β和IL-8的分子特征进行研究，为推进对该动物功能性免疫反应的研究提供了必要的基础信息，并有可能将它们作为重组蛋白用于调控免疫反应。
