Ma Lin, Li Zaiyi, Xi Sujuan, Guo Quan, Zhao Panlin, Li Weihao, Ai Jing, Chen Xiaoxuan
The Reproductive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University Shenzhen 510000, Guangdong Province, China.
The Reproductive Medicine Center, The Third Affiliated Hospital, Sun Yat-sen University Guangzhou 510630, Guangdong Province, China.
Am J Transl Res. 2020 Sep 15;12(9):5741-5751. eCollection 2020.
Tubal ectopic pregnancy (TEP) remains the most common cause of maternal morbidity and mortality in the early months of pregnancy. The aim of this study is to perform the correlation between PROKRs and pro-inflammatory genes and explore the role of novel genes in pathogenesis of TEP.
Here, quantitative real time PCR and immunohistochemistry were used to assess the expression of the novel genes in 120 TEP patients and 30 age-matched non-TEP patients. The correlation between PROKRs and pro-inflammatory genes were analyzed by Pearson correlation coefficient. Univariate and multivariate Cox regression analyses were used to assess the risk prediction rate of novel genes. Receiver operating characteristic was used to assess the performance of our model.
PROKRs (PROKR1 and PROKR2) and pro-inflammatory genes (TNF-α, IL-6, and IL-8) expression levels significantly enhanced in TEP patients, and significantly positive correlation with pro-inflammatory genes for PROKRs. A multivariate Cox regression analysis demonstrated that 2 genes (PROKR2 and IL8) had significant diagnostic value, which were associated with the occurrence and development of TEP.
Our data further denote that dysregulation of PROKR2 and IL-8 were risk factor and played an important role in the pathogenesis of TEP.
输卵管异位妊娠（TEP）仍然是妊娠早期孕产妇发病和死亡的最常见原因。本研究的目的是进行PROKRs与促炎基因之间的相关性分析，并探讨新基因在TEP发病机制中的作用。
在此，采用定量实时PCR和免疫组织化学方法评估120例TEP患者和30例年龄匹配的非TEP患者中这些新基因的表达情况。通过Pearson相关系数分析PROKRs与促炎基因之间的相关性。采用单因素和多因素Cox回归分析评估新基因的风险预测率。使用受试者工作特征曲线评估我们模型的性能。
TEP患者中PROKRs（PROKR1和PROKR2）和促炎基因（TNF-α、IL-6和IL-8）的表达水平显著升高，且PROKRs与促炎基因呈显著正相关。多因素Cox回归分析表明，2个基因（PROKR2和IL8）具有显著的诊断价值，与TEP的发生发展相关。
我们的数据进一步表明，PROKR2和IL-8的失调是危险因素，在TEP的发病机制中起重要作用。
