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用N-乙酰胞壁酸抗体对角贝(扁卷螺科,肺螺亚纲)血细胞亚群进行免疫检测

Immunodetection of haemocyte subpopulations by N-acetylmuramic acid antibody in Planorbarius corneus (L.) (Gastropoda, Pulmonata).

作者信息

Ottaviani E, Montagnani G

机构信息

Department of Animal Biology, University of Modena, Italy.

出版信息

Histochem J. 1989 Nov;21(11):675-8. doi: 10.1007/BF01002488.

DOI:10.1007/BF01002488
PMID:2684926
Abstract

The cell subpopulations in the haemolymph of Planorbarius corneus were distinguished by means of flow cytometry. An antibody against N-acetylmuramic acid was prepared and used as a cellular marker to recognize the cell types forming the subpopulations. The spreading haemocytes showed a positive reaction for anti-N-acetylmuramic acid; round haemocytes gave a negative reaction.

摘要

通过流式细胞术区分了角贝血淋巴中的细胞亚群。制备了一种抗N-乙酰胞壁酸的抗体,并将其用作细胞标记物来识别构成亚群的细胞类型。铺展型血细胞对抗N-乙酰胞壁酸呈阳性反应;圆形血细胞呈阴性反应。

相似文献

1
Immunodetection of haemocyte subpopulations by N-acetylmuramic acid antibody in Planorbarius corneus (L.) (Gastropoda, Pulmonata).用N-乙酰胞壁酸抗体对角贝(扁卷螺科,肺螺亚纲)血细胞亚群进行免疫检测
Histochem J. 1989 Nov;21(11):675-8. doi: 10.1007/BF01002488.
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引用本文的文献

1
Identification of muramyl derivatives in Mollusca Gastropoda tissue.腹足纲软体动物组织中胞壁酰衍生物的鉴定。
Histochemistry. 1991;96(3):209-13. doi: 10.1007/BF00271539.
2
N-acetylmuramic acid in Mollusca Gastropoda: a histochemical and immunocytochemical study.腹足纲软体动物中的N-乙酰胞壁酸:一项组织化学和免疫细胞化学研究。
Histochem J. 1990 Sep;22(9):475-9. doi: 10.1007/BF01007231.

本文引用的文献

1
Lipid composition in ganglia of Mollusca.
J Neurochem. 1981 Mar;36(3):821-5. doi: 10.1111/j.1471-4159.1981.tb01667.x.
2
The blood cells of the freshwater snail Planorbis corneus (Gastropoda, Pulmonata).
Dev Comp Immunol. 1983 Spring;7(2):209-16. doi: 10.1016/0145-305x(83)90002-2.
3
Fluorescence-activated cell sorting.荧光激活细胞分选
Sci Am. 1976 Mar;234(3):108-17. doi: 10.1038/scientificamerican0376-108.