腹足纲软体动物组织中胞壁酰衍生物的鉴定。

Identification of muramyl derivatives in Mollusca Gastropoda tissue.

作者信息

Menghi G, Ottaviani E, Accili D, Bolognani Fantin A M

机构信息

Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.

出版信息

Histochemistry. 1991;96(3):209-13. doi: 10.1007/BF00271539.

DOI:10.1007/BF00271539

PMID:1917577

Abstract

Previous findings have demonstrated the presence of muramic acid and the lack of sialic acid in gastropod glycoconjugates from different tissues. The present study investigated the composition of muramyl derivatives in Mollusca Gastropoda tissue from the foot, mantle and periesophageal ganglia, using HRP-labeled lectins (LTA, UEA I, GSA IB4, GSA II, DBA, SBA, RCA II, WGA, PNA, ConA) and glycosidase digestion (neuraminidase, lysozyme, alpha-L-fucosidase, beta-N-acetylglucosaminidase, alpha-N-acetylgalactosaminidase). Muramyl derivatives from the tissue examined showed some differences related to the composition of the terminal disaccharides. Indeed, foot and mantle mucocytes exhibited muramic acid in a terminal position, linked to (subterminal) N-acetylgalactosamine, whereas in neuron cells muramic acid was present in an internal position and linked to N-acetylglucosamine. Diversities also occurred between foot and mantle mucocytes with respect to the receptor sugar for penultimate N-acetylgalactosamine.

摘要

先前的研究结果表明，来自不同组织的腹足纲动物糖缀合物中存在胞壁酸且缺乏唾液酸。本研究使用辣根过氧化物酶标记的凝集素（LTA、UEA I、GSA IB4、GSA II、DBA、SBA、RCA II、WGA、PNA、ConA）和糖苷酶消化（神经氨酸酶、溶菌酶、α-L-岩藻糖苷酶、β-N-乙酰氨基葡萄糖苷酶、α-N-乙酰半乳糖苷酶），研究了腹足纲软体动物足部、外套膜和食管周围神经节组织中胞壁酰衍生物的组成。所检测组织中的胞壁酰衍生物在末端二糖组成方面存在一些差异。实际上，足部和外套膜的黏液细胞在末端位置显示有胞壁酸，与（亚末端）N-乙酰半乳糖胺相连，而在神经细胞中，胞壁酸存在于内部位置并与N-乙酰氨基葡萄糖相连。足部和外套膜黏液细胞在倒数第二个N-乙酰半乳糖胺的受体糖方面也存在差异。