Sun Yang, Yang Chao, Chen Jieying, Song Xin, Li Zhen, Duan Minlan, Li Jianglin, Hu Xiaoxiao, Wu Kuangpei, Yan Guobei, Yang Cai, Liu Jing, Tan Weihong, Ye Mao
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Molecular Engineering for Theranostics, Hunan University, Changsha, Hunan, China.
College of Life and Environmental Sciences, Gannan Normal University, Ganzhou, Jiangxi, China.
J Cell Mol Med. 2016 Apr;20(4):698-709. doi: 10.1111/jcmm.12759. Epub 2016 Feb 5.
WD-repeat protein 79 (WDR79), a member of the WD-repeat protein family, acts as a scaffold protein, participating in telomerase assembly, Cajal body formation and DNA double-strand break repair. Here, we first report that WDR79 is frequently overexpressed in cell lines and tissues derived from non-small cell lung cancer (NSCLC). Knockdown of WDR79 significantly inhibited the proliferation of NSCLC cells in vitro and in vivo by inducing cell cycle arrest and apoptosis. WD-repeat protein 79 -induced cell cycle arrest at the G0/G1 phase was associated with the expression of G0/G1-related cyclins and cyclin-dependent kinase complexes. We also provide evidence that WDR79 knockdown induces apoptosis via a mitochondrial pathway. Collectively, these results suggest that WDR79 is involved in the tumorigenesis of NSCLC and is a potential novel diagnostic marker and therapeutic target for NSCLC.
WD重复蛋白79（WDR79）是WD重复蛋白家族的成员，作为一种支架蛋白，参与端粒酶组装、卡哈尔体形成和DNA双链断裂修复。在此，我们首次报道WDR79在非小细胞肺癌（NSCLC）来源的细胞系和组织中经常过度表达。敲低WDR79可通过诱导细胞周期停滞和凋亡在体外和体内显著抑制NSCLC细胞的增殖。WD重复蛋白79诱导的细胞周期在G0/G1期停滞与G0/G1相关细胞周期蛋白和细胞周期蛋白依赖性激酶复合物的表达有关。我们还提供证据表明，敲低WDR79可通过线粒体途径诱导凋亡。总体而言，这些结果表明WDR79参与NSCLC的肿瘤发生，是NSCLC潜在的新型诊断标志物和治疗靶点。
