[2014年广东省食源性副溶血性弧菌分离株的病原学特征]

[Etiologic characteristics of food-borne Vibrio parahaemolyticus strains isolated in Guangdong, 2014].

作者信息

Li Baisheng, Tan Hailing, Ke Bixia, He Dongmei, Ke Changwen, Zhang Yonghui

机构信息

Institute of Pathogenic Microbiology Bacteriology Laboratory, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.

Institute of Pathogenic Microbiology Bacteriology Laboratory, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China; Email:

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2015 Nov;36(11):1283-7.

PMID:26850252

Abstract

OBJECTIVE

To investigate the serotypes, antimicrobial resistance, virulence genes and molecular characteristics of food-borne Vibrio parahaemolyticus isolated in Guangdong province in 2014.

METHODS

Sixty V. parahaemolyticus strains were tested in this study. The serotyping and antibiotic resistance test were conducted, while the tdh and trh gens of the strains were detected with conventional PCR, and pulsed field gel electrophoresis (PFGE) and multiple locus sequence typing (MLST) were conducted too.

RESULTS

The 60 strains belonged to 13 serotypes, the major serotypes included O3: K6, O4: K8, O1: K36 and O4: KUT. The antibiotic resistance test indicated the isolates were highly resistant to ampicillin (100.0%), sulfonamides (43.3%) and cefalotin (28.3%). Up to 56.7%(34/60) of the strains were resistant to two or more antibiotics, and 2 strains showed resistance to three antibiotics. The virulence gene detection indicated that 63.3% (38/60) of the strains carried tdh⁺ trh⁻, while only 1 strain carried tdh⁺ trh⁺. The 60 strains digested by NotI belonged to 48 different PFGE patterns and 3 clusters. The cluster B included the strains isolated from sporadic food borne cases with serotype of O3: K6 and similarity of 62.6%-100.0%. The cluster C included O4: K8 strains with the PFGE pattern similarity of 56.7%-62.5%. The MLST indicated that the 60 strains had 26 sequence types (STs). The ST-3 was predominant, including 33 strains (serotypes O3: K6 and O1 :K36). The four O4: K8 strains formed another predominant colony, which was different from ST-3.

CONCLUSION

The etiologic characteristics of V. parahaemolyticus varied, which might be one of the reasons for high incidence of food-borne V. parahaemolyticus infection in Guangdong. The molecular characteristics of O4: K8 strain were different from the other predominant serotypes. Close attention should be paid to the possible outbreak caused by O4: K8 strain in this area.

摘要

目的

调查2014年广东省分离的食源性副溶血性弧菌的血清型、耐药性、毒力基因及分子特征。

方法

本研究对60株副溶血性弧菌进行检测。进行血清分型和药敏试验，同时采用常规PCR检测菌株的tdh和trh基因，并进行脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）。

结果

60株菌属于13个血清型，主要血清型包括O3:K6、O4:K8、O1:K36和O4:KUT。药敏试验表明，分离株对氨苄西林（100.0%）、磺胺类药物（43.3%）和头孢噻吩（28.3%）高度耐药。高达56.7%（34/60）的菌株对两种或两种以上抗生素耐药，2株对三种抗生素耐药。毒力基因检测表明，63.3%（38/60）的菌株携带tdh⁺trh⁻，而只有1株携带tdh⁺trh⁺。用NotI酶切的60株菌属于48种不同的PFGE图谱和3个簇。B簇包括从散发食源性病例中分离的血清型为O3:K6且相似度为62.6%-100.0%的菌株。C簇包括PFGE图谱相似度为56.7%-62.5%的O4:K8菌株。MLST表明60株菌有26种序列型（STs）。ST-3为主型，包括33株（血清型O3:K6和O1:K36）。4株O4:K8菌株形成另一个优势菌群，与ST-3不同。