[中国土拉热弗朗西斯菌全北区亚种的遗传多样性]

[Genetic diversity of Francisella tularensis subsp. holarctica in China].

作者信息

Wang Yanhua, Peng Yao, Xia Lianxu

机构信息

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Email:

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2015 Dec;36(12):1410-4.

PMID:26850402

Abstract

OBJECTIVE

To explore the genetic relationship between the Chinese and the foreign species of Francisella tularensis.

METHODS

Based on our own findings and from the literature, 17 SNP, 4 INDEL, and 12 VNTR were selected for phylogenetic analysis on 39 strains of F.tularensis, including 10 strains of Chinese F. tularensis and 29 strains of foreign F. tularensis that had been sequenced and published. SNP-INDEL and MLVA were used for the separation and combination.

RESULTS

Data from the combined analysis indicated that 3 strains of Chinese F. tularensis with Japanese FSC022 were assigned to B5; 3 strains, with Swedish FSC200 to B1; 3 strains with American OSU18 to B2 and 1 strain with French FTNF002-00, German F92, and American OR96246 to B4, respectively. 10 strains of Chinese F. tularensis were assigned to 4 clades and the result demonstrated a wide diversity of F. tularensis subsp.holarctica in China.

CONCLUSION

A set of simple and robust typing tools for F. tularensis subsp.holarctica were established in this study. Based on the results, F. tularensis subsp.holarctica might have had its origins in Asia.

摘要

目的

探讨中国与国外土拉弗朗西斯菌物种之间的遗传关系。

方法

基于我们自己的研究结果并从文献中选取17个单核苷酸多态性（SNP）、4个插入缺失（INDEL）和12个可变数目串联重复序列（VNTR），对39株土拉弗朗西斯菌进行系统发育分析，其中包括10株已测序并发表的中国土拉弗朗西斯菌和29株国外土拉弗朗西斯菌。采用SNP-INDEL和多位点可变数目串联重复序列分析（MLVA）进行分型组合。