中国和其他国家的土拉弗朗西斯菌菌株之间的遗传关系。

Genetic relationship between Francisella tularensis strains from China and from other countries.

机构信息

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

出版信息

Biomed Environ Sci. 2011 Jun;24(3):310-4. doi: 10.3967/0895-3988.2011.03.015.

DOI:10.3967/0895-3988.2011.03.015

PMID:21784318

Abstract

OBJECTIVE

To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries.

METHODS

Ten strains of F. tularensis isolated from China were amplified by using typing primers C1/C4 and RD1. On the basis of the lengths of the polymerase chain reaction (PCR) products, it was concluded that these strains of F. tularensis belonged to the same subspecies. At the same time, the fopA, tul4, and 16S rRNA genes of the 10 strains were amplified, and a three-gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0.

RESULTS

The 10 strains of F. tularensis from China were all identified as belonging to subspecies holarctica (type B). We found no direct relationship between the genotypes of F. tularensis subsp. holarctica and the geographical area from where they were isolated.

CONCLUSION

The F. tularensis strains isolated from North China mainly belong to subspecies holarctica (type B). The strains of F. tularensis subsp. holarctica from China may have evolved earlier than those from Europe and North America.

摘要

目的

研究来自中国的土拉弗朗西斯菌亚种类型，并探讨中国和其他国家的土拉弗朗西斯菌菌株之间的遗传关系。

方法

使用分型引物 C1/C4 和 RD1 对 10 株来自中国的土拉弗朗西斯菌进行扩增。根据聚合酶链反应（PCR）产物的长度，得出这些土拉弗朗西斯菌菌株属于同一亚种。同时，扩增 10 株菌的 fopA、tul4 和 16S rRNA 基因，并使用 Molecular Evolutionary Genetics Analysis 软件版本 4.0 进行基于三基因的系统发育分析。