周期性拉伸通过诱导活性氧生成增强人腰椎黄韧带细胞凋亡。
Cyclic stretch enhances apoptosis in human lumbar ligamentum flavum cells via the induction of reactive oxygen species generation.
作者信息
Chen Jianwei, Liu Zude, Zhong Guibin, Li Zhanchun, Qian Lie, Li Xinfeng, Chen Bin, Lao Lifeng, Wang Hantao
机构信息
a Department of Orthopedics, Renji Hospital, School of Medicine , Shanghai JiaoTong University , Shanghai , People's Republic of China.
出版信息
J Spinal Cord Med. 2016 Jul;39(4):450-4. doi: 10.1080/10790268.2016.1141470. Epub 2016 Feb 10.
Abstract
OBJECTIVE
The lumbar ligamentum flavum (LF) is an important part of the spine to maintain the stability of the spine. In this study we aimed to examine whether mechanical force by cyclic stretch could induce apoptosis in human LF cells and investigate the underlying mechanism.
METHODS
LF cells were isolated from six young patients undergoing spinal surgery and then cultured in vitro. LF cells were subjected to cyclic stretch and the poptosis was detected by flow cytometry. The level of intracellular reactive oxygen species (ROS) and caspase-9 activity were measured.
RESULTS
Cyclic stretch at a frequency of 0.5 Hz with 20% elongation induced the apoptosis of human LF cells in vitro, and this was correlated with increased ROS generation and activation of caspase-9.
CONCLUSION
Our study suggests that cyclic stretch-induced apoptosis in human LF cells may be mediated by ROS generation and the activation of caspase-9.
摘要
目的
腰椎黄韧带（LF）是维持脊柱稳定性的脊柱重要组成部分。在本研究中，我们旨在研究周期性拉伸产生的机械力是否能诱导人LF细胞凋亡，并探究其潜在机制。
方法
从6例接受脊柱手术的年轻患者中分离出LF细胞，然后进行体外培养。对LF细胞进行周期性拉伸，并通过流式细胞术检测细胞凋亡情况。测量细胞内活性氧（ROS）水平和半胱天冬酶-9活性。
结果
在0.5Hz频率、20%伸长率的周期性拉伸诱导了体外培养的人LF细胞凋亡，且这与ROS生成增加和半胱天冬酶-9激活相关。
结论
我们的研究表明，周期性拉伸诱导人LF细胞凋亡可能是由ROS生成和半胱天冬酶-9激活介导的。
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