MEK/ERK 信号通路在 SW620 细胞系凋亡中的作用及白藜芦醇的抑制作用。
MEK/ERK signaling pathway in apoptosis of SW620 cell line and inhibition effect of resveratrol.
机构信息
Jingzhou Central Hospital, Wuhan City, Hubei Province, China.
Jingzhou Central Hospital, Wuhan City, Hubei Province, China.
出版信息
Asian Pac J Trop Med. 2016 Jan;9(1):49-53. doi: 10.1016/j.apjtm.2015.12.010. Epub 2015 Dec 19.
OBJECTIVE
To study the involvement of MAPK MEK/ERK signaling transduction pathway in the apoptosis process of SW620 tumor cell line and the inhibition effect of resveratrol.
METHODS
SW620 cell lines were divided into 5 groups, namely, control group, PD98059 group, low-dose resveratrol group, mid-dose resveratrol group and high-dose resveratrol group. The inhibition rate of cell proliferation was detected by MTT method. The expression of apoptotic molecules and MEK/ERK signaling pathway related proteins were assayed by real-time PCR and Western blotting.
RESULTS
Compared with control group, the proliferation of cells treated with resveratrol was significantly inhibited. In the case of apoptotic molecules, the expression of Bax, Caspase 3 and Caspase 9 was increased significantly while the expression of anti-apoptotic molecule Bcl2 was decreased significantly in resveratrol groups with a dose-dependent manner. In the case of molecules in MEK/ERK signaling pathway, the expression of Ras, Raf, MEK and ERK1/2 was decreased significantly in resveratrol groups with a dose-dependent manner.
CONCLUSIONS
PD98059 and resveratrol can effectively inhibit the proliferation of SW620 through inhibiting the MEK/ERK signaling pathway.
目的
研究 MAPK MEK/ERK 信号转导通路在 SW620 肿瘤细胞系凋亡过程中的作用及白藜芦醇的抑制作用。
方法
将 SW620 细胞株分为 5 组，即对照组、PD98059 组、低剂量白藜芦醇组、中剂量白藜芦醇组和高剂量白藜芦醇组。采用 MTT 法检测细胞增殖抑制率，实时 PCR 和 Western blot 法检测凋亡分子和 MEK/ERK 信号通路相关蛋白的表达。
结果
与对照组相比，白藜芦醇处理后的细胞增殖明显受到抑制。在凋亡分子方面，白藜芦醇组 Bax、Caspase 3 和 Caspase 9 的表达明显增加，而抗凋亡分子 Bcl2 的表达明显减少，呈剂量依赖性。在 MEK/ERK 信号通路分子方面，白藜芦醇组 Ras、Raf、MEK 和 ERK1/2 的表达明显减少，呈剂量依赖性。
结论
PD98059 和白藜芦醇通过抑制 MEK/ERK 信号通路有效抑制 SW620 的增殖。
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