Zhao Yan, Wu Jun-Wei, Wang Yan, Zhao Jin-Liang
Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China.
Biochem Biophys Res Commun. 2016 Feb 26;471(1):26-33. doi: 10.1016/j.bbrc.2016.02.007. Epub 2016 Feb 10.
MicroRNAs (miRNAs) are a class of short, evolutionary conserved non-coding RNA molecules, which are shown as the key regulators of many biological functions. External stress can alter miRNA expression levels, thereby changing the expression of mRNA target genes. Here, we show that miR-21 is involved in the regulation of alkalinity tolerance in Nile tilapia. Alkalinity stress results in a marked reduction in miR-21 levels. miR-21 loss of function could affect ion balance regulation, ROS production, and antioxidant enzyme activity in vivo. Moreover, miR-21 knockdown protects cell against alkalinity stress-induced injury in vitro. miR-21 directly regulates VEGFB and VEGFC expression by targeting the 3'-untranslated regions (UTRs) of their mRNAs, and inhibition of miR-21 significantly increases the levels of VEGFB and VEGFC expression in vivo. Taken together, our study reveals that miR-21 knockdown plays a protective role in alkalinity tolerance in tilapia.
微小RNA（miRNA）是一类短小的、进化上保守的非编码RNA分子，它们是许多生物学功能的关键调节因子。外部应激可改变miRNA的表达水平，从而改变mRNA靶基因的表达。在此，我们表明miR-21参与尼罗罗非鱼耐碱性的调节。碱度应激导致miR-21水平显著降低。miR-21功能丧失会影响体内离子平衡调节、活性氧产生和抗氧化酶活性。此外，敲低miR-21可在体外保护细胞免受碱度应激诱导的损伤。miR-21通过靶向VEGFB和VEGFC mRNA的3'-非翻译区（UTR）直接调节它们的表达，抑制miR-21可显著提高体内VEGFB和VEGFC的表达水平。综上所述，我们的研究表明敲低miR-21在罗非鱼耐碱性方面发挥保护作用。
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