Su Huanhuan, Fan Jiajia, Ma Dongmei, Zhu Huaping
Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Front Genet. 2021 Apr 6;12:526277. doi: 10.3389/fgene.2021.526277. eCollection 2021.
Researchers have increasingly suggested that microRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression and protein translation in organs and respond to abiotic and biotic stressors. To understand the function of miRNAs in osmotic stress regulation of the gills of hybrid tilapia ( × ), high-throughput Illumina deep sequencing technology was used to investigate the expression profiles of miRNAs under salinity stress (S, 25‰), alkalinity stress (A, 4‰) and salinity-alkalinity stress (SA, S: 15‰, A: 4‰) challenges. The results showed that 31, 41, and 27 upregulated and 33, 42, and 40 downregulated miRNAs ( < 0.05) were identified in the salt stress, alkali stress, and saline-alkali stress group, respectively, which were compared with those in the control group (C). Fourteen significantly differently expressed miRNAs were selected randomly and then validated by a quantitative polymerase chain reaction. On the basis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, genes related to osmoregulation and biosynthesis were enriched in the three types of osmotic stress. In addition, three miRNAs and three predicted target genes were chosen to conduct a quantitative polymerase chain reaction in the hybrid tilapia and its parents during 96-h osmotic stress. Differential expression patterns of miRNAs provided the basis for research data to further investigate the miRNA-modulating networks in osmoregulation of teleost.
研究人员越来越多地指出，微小RNA（miRNA）是一类小的非编码RNA，它们在转录后调节器官中的基因表达和蛋白质翻译，并对非生物和生物胁迫作出反应。为了解miRNA在杂交罗非鱼（×）鳃的渗透胁迫调节中的功能，采用高通量Illumina深度测序技术研究了盐度胁迫（S，25‰）、碱度胁迫（A，4‰）和盐度-碱度胁迫（SA，S：15‰，A：4‰）挑战下miRNA的表达谱。结果表明，与对照组（C）相比，在盐胁迫、碱胁迫和盐碱胁迫组中分别鉴定出31、41和27个上调的miRNA以及33、42和40个下调的miRNA（<0.05）。随机选择14个差异显著的miRNA，然后通过定量聚合酶链反应进行验证。基于基因本体论和京都基因与基因组百科全书通路分析，与渗透调节和生物合成相关的基因在三种类型的渗透胁迫中富集。此外，选择三个miRNA和三个预测的靶基因在杂交罗非鱼及其亲本的96小时渗透胁迫期间进行定量聚合酶链反应。miRNA的差异表达模式为进一步研究硬骨鱼渗透调节中的miRNA调控网络提供了研究数据基础。
