Dhakal Dipesh, Kumar Jha Amit, Pokhrel Anaya, Shrestha Anil, Sohng Jae Kyung
Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
Curr Protoc Microbiol. 2016 Feb 8;40:10F.2.1-10F.2.18. doi: 10.1002/9780471729259.mc10f02s40.
Nocardia spp. are aerobic, Gram-positive, catalase positive, and non-motile actinomycetes. They are associated with human infections. However, some species produce important natural products, degrade toxic chemicals, and are involved in biotransformation of valuable products. The lack of robust genetic tools has hindered detailed studies and advanced research. This unit describes the major genetic engineering approaches using Nocardia sp. CS682 as a prototype. These methods will certainly help in understanding the basis of their pathogenicity as well as biosynthetic and biotransforming abilities. It can be expected that knowledge of the biochemistry behind their pathogenicity will be crucial in developing effective treatment strategies. These genetic tools can be utilized to develop rational metabolic engineering approaches for crafting host strains with higher production or biotransformation ability.
诺卡氏菌属是需氧、革兰氏阳性、过氧化氢酶阳性且无运动性的放线菌。它们与人类感染有关。然而，一些物种能产生重要的天然产物，降解有毒化学物质，并参与有价值产品的生物转化。缺乏强大的遗传工具阻碍了详细研究和深入探索。本单元以诺卡氏菌CS682为原型描述了主要的基因工程方法。这些方法肯定有助于理解它们的致病性以及生物合成和生物转化能力的基础。可以预期，了解其致病性背后的生物化学知识对于制定有效的治疗策略至关重要。这些遗传工具可用于开发合理的代谢工程方法，以构建具有更高生产或生物转化能力的宿主菌株。
