Dhakal Dipesh, Sohng Jae Kyung
Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
Curr Protoc Microbiol. 2015 Nov 3;39:10F.1.1-10F.1.8. doi: 10.1002/9780471729259.mc10f01s39.
Nocardia spp. are aerobic, Gram-positive, catalase-positive, non-motile actinomycetes. Various species of the genus Nocardia have attracted attention due to their detrimental effects on human health. Recent discoveries, however, have exposed their importance as producers of bioactive compounds and degraders of complex organic compounds, as well as their involvement in biotransformation into valuable products. This unit includes general protocols for the laboratory maintenance of Nocardia spp., including growth in liquid medium, growth on solid agar, and long-term storage. Nocardia sp. CS682 (KCTC11297BP), isolated from soil collected in Jeonnam, Korea, is used as a prototype for explaining the considerations for efficient laboratory maintenance of Nocardia spp.
诺卡氏菌属是需氧、革兰氏阳性、过氧化氢酶阳性、不运动的放线菌。诺卡氏菌属的各种菌种因其对人类健康的有害影响而受到关注。然而，最近的发现揭示了它们作为生物活性化合物生产者、复杂有机化合物降解者的重要性，以及它们参与生物转化为有价值产品的情况。本单元包括诺卡氏菌属实验室保存的通用方案，包括在液体培养基中生长、在固体琼脂上生长以及长期保存。从韩国全罗南道采集的土壤中分离出的诺卡氏菌CS682（KCTC11297BP）用作解释诺卡氏菌属高效实验室保存注意事项的原型。
