Kasloff Samantha B, Weingartl Hana M
Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba, Canada.
Virology. 2016 Mar;490:91-8. doi: 10.1016/j.virol.2016.01.006. Epub 2016 Feb 6.
The importance of pigs in interspecies transmission of influenza A viruses has been repeatedly demonstrated over the last century. Eleven influenza A viruses from avian, human and swine hosts were evaluated for replication phenotypes at three physiologically relevant temperatures (41°C, 37°C, 33°C) in an immortalized swine pulmonary alveolar macrophage cell line (IPAM 3D4/31) to determine whether this system would allow for their efficient replication. All isolates replicated well in IPAMs at 37°C while clear distinctions were observed at 41°C and 33°C, correlating to species of origin of the PB2, reflected in distinct amino acid residue profiles rather than in one particular PB2 residue. A strong TNF-α response was induced by some mammalian but not avian IAVs, while other selected cytokines remained below detection levels. Porcine IPAMs represent a natural host cell model for influenza virus replication where the only condition requiring modification for optimal IAV replication, regardless of virus origin.
在过去的一个世纪里，猪在甲型流感病毒跨物种传播中的重要性已得到反复证实。对来自禽类、人类和猪宿主的11种甲型流感病毒在永生化猪肺泡巨噬细胞系（IPAM 3D4/31）中的三种生理相关温度（41°C、37°C、33°C）下的复制表型进行了评估，以确定该系统是否能使其有效复制。所有分离株在37°C时在IPAM中复制良好，而在41°C和33°C时观察到明显差异，这与PB2的来源物种相关，体现在不同的氨基酸残基谱中，而非某一个特定的PB2残基。一些哺乳动物的甲型流感病毒能诱导强烈的TNF-α反应，而禽类的甲型流感病毒则不能，而其他选定的细胞因子仍低于检测水平。猪IPAM代表了一种用于流感病毒复制的天然宿主细胞模型，无论病毒来源如何，唯一需要调整的条件是为了实现最佳的甲型流感病毒复制。
