State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST), Beijing, 100083, P. R. China.
Small. 2016 Apr 6;12(13):1770-8. doi: 10.1002/smll.201503946. Epub 2016 Feb 9.
As a physical cue for controlling the fate of stem cells, surface nanotopography has attracted much attention to improve the integration between implants and local host tissues and cells. A biocompatible surface TiO2 nanorod array is proposed to regulate the fate of bone marrow derived mesenchymal stem cells (MSCs). TiO2 substrates with different surface nanotopographies: a TiO2 nanorod array and a polished TiO2 ceramic are built by hydrothermal and sintering processes, respectively. The assessment of morphology, viability, gene expression, and protein characterization of the MSCs cultured on the different TiO2 substrates proves that a TiO2 nanorod array promotes the osteogenic differentiation of MSCs, while a TiO2 ceramic with a smooth surface suppresses it. Periodically assembled TiO2 nanorod array stripes on the smooth TiO2 ceramic are constructed by a combination of microfabrication and a chemical synthesis process, which realizes the location-committed osteogenic differentiation of MSCs. A route to control the differentiation of MSCs by a nanostructured surface, which can also control the location and direction of MSCs on the surface of biomaterials with micro-nano scale surface engineering, is demonstrated.
作为控制干细胞命运的物理线索，表面纳米形貌引起了人们极大的关注，以改善植入物与局部宿主组织和细胞的整合。提出了一种生物相容性表面 TiO2 纳米棒阵列来调节骨髓间充质干细胞（MSCs）的命运。通过水热和烧结工艺分别构建了具有不同表面纳米形貌的 TiO2 基底：TiO2 纳米棒阵列和抛光 TiO2 陶瓷。对不同 TiO2 基底上培养的 MSCs 的形态、活力、基因表达和蛋白质特性进行评估，证明 TiO2 纳米棒阵列促进了 MSCs 的成骨分化，而具有光滑表面的 TiO2 陶瓷则抑制了其分化。通过微加工和化学合成工艺的组合，在光滑的 TiO2 陶瓷上构建周期性组装的 TiO2 纳米棒阵列条带，实现了 MSCs 的位置特异性成骨分化。通过纳米结构表面控制 MSCs 分化的途径得到了证明，该途径还可以通过微纳尺度表面工程控制 MSCs 在生物材料表面的位置和方向。
