Li Zhi, Qiu Jichuan, Du Ling Qian, Jia Lu, Liu Hong, Ge Shaohua
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan 250012, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
Center of Bio&Micro/Nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University, Jinan 250010, China.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:684-691. doi: 10.1016/j.msec.2017.03.148. Epub 2017 Mar 18.
Nanostructure coating on titanium (Ti) implants is well known as a cue for directing osteoblast behavior and function. However, effects of nanostructure coatings on dental stem cells have been rarely explored. In this work, assembled TiO nanorod arrays (TNRs) were fabricated on the polished Ti substrates using hydrothermal and sintering methods. The adhesion, morphology, proliferation and osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) seeded onto TNRs substrates were evaluated. Ti substrates were used as control. Rougher TNRs showed better hydrophilicity and protein adsorption capacity compared with Ti control. When seeded on TNRs substrates, PDLSCs exhibited more stretched morphology and higher proliferation rate. Cytoskeletal F-actin expression was markedly promoted for PDLSCs cultured on TNRs substrates under osteogenic induction. Alkaline phosphatase (ALP) activity and mineral deposition were also enhanced by TNRs. Moreover, osteogenesis-related markers of ALP, runt related transcription factor 2 (Runx2) and osteopontin (OPN) of PDLSCs cultured on TNRs substrates were significantly up-regulated at both gene and protein levels when compared to Ti substrates. In conclusion, the unique structure of TNRs provided a biocompatible platform for modulating morphology and function of PDLSCs. The promotion of osteogenic differentiation indicated that the surface modification of implants with TNRs may improve the osteogenic activity of implants and the bone-implant integration in future clinical applications.
钛（Ti）植入物上的纳米结构涂层作为引导成骨细胞行为和功能的线索已广为人知。然而，纳米结构涂层对牙干细胞的影响却鲜有研究。在这项工作中，采用水热法和烧结法在抛光的Ti基体上制备了组装TiO纳米棒阵列（TNRs）。评估了接种在TNRs基体上的人牙周膜干细胞（PDLSCs）的黏附、形态、增殖和成骨分化情况。Ti基体用作对照。与Ti对照相比，更粗糙的TNRs表现出更好的亲水性和蛋白质吸附能力。当接种在TNRs基体上时，PDLSCs表现出更伸展的形态和更高的增殖率。在成骨诱导下，培养在TNRs基体上的PDLSCs的细胞骨架F-肌动蛋白表达明显增强。TNRs还增强了碱性磷酸酶（ALP）活性和矿物质沉积。此外，与Ti基体相比，培养在TNRs基体上的PDLSCs的ALP、 runt相关转录因子2（Runx2）和骨桥蛋白（OPN）等成骨相关标志物在基因和蛋白质水平均显著上调。总之，TNRs的独特结构为调节PDLSCs的形态和功能提供了一个生物相容性平台。成骨分化的促进表明，用TNRs对植入物进行表面修饰可能会在未来的临床应用中提高植入物的成骨活性和骨-植入物整合。
