Chutani Preeti, Sharma Krishna Kant
Laboratory of Enzymology and Recombinant DNA Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
Bioprocess Biosyst Eng. 2016 May;39(5):747-58. doi: 10.1007/s00449-016-1555-3. Epub 2016 Feb 8.
Sixty fungal cultures were isolated from agricultural soil, industrial soil, forest canopy soil having decomposed leaf litter and compost samples collected from different regions of India. Fifteen fungal cultures were selected qualitatively for the production of xylanase and cellulases and were identified employing ITS, NS and MNS primers. The enzyme cocktail consisting of 3811 IU g(-1) of xylanase and 9.9 IU g(-1) of cellulase from Trichoderma longibrachiatum MDU-6 was selected quantitatively for the deinking of diverse paper wastes. The enzyme production increased two fold when produced at tray level in comparison with flasks. The enzyme cocktail was effective in the deinking of old newspaper samples with significant removal of chromophores, phenolics and hydrophobic compounds and less sugar loss. While in case of examination papers and laser printed papers, ink removal was not very significant. Moreover, the sugar loss was significantly high in case of examination papers. The deinking results were further confirmed with FTIR analysis. Deinked newspaper pulp sample shows brightness of 52%, which was 9.6% high than its control sample. The ERIC value for deinked newspaper pulp was found to be 655.9 ppm. Thereafter, the deinked newspaper pulp was examined under light microscope after differential staining with safranin and malachite green and also examined under scanning and transmission electron microscope, which revealed fibrillation and perforation.
从印度不同地区采集的农业土壤、工业土壤、带有腐烂落叶的森林冠层土壤和堆肥样品中分离出60种真菌培养物。定性选择了15种真菌培养物用于木聚糖酶和纤维素酶的生产，并使用ITS、NS和MNS引物进行鉴定。定量选择了由长枝木霉MDU-6产生的含有3811 IU g(-1)木聚糖酶和9.9 IU g(-1)纤维素酶的酶混合物用于多种废纸的脱墨。与在烧瓶中生产相比，在托盘水平生产时酶产量提高了两倍。该酶混合物对旧报纸样品的脱墨有效，能显著去除发色团、酚类和疏水化合物，且糖损失较少。而对于试卷和激光打印纸，油墨去除效果不太显著。此外，试卷的糖损失显著较高。脱墨结果通过傅里叶变换红外光谱分析进一步得到证实。脱墨后的报纸纸浆样品亮度为52%，比其对照样品高9.6%。脱墨报纸纸浆的ERIC值为655.9 ppm。此后，用番红和孔雀石绿进行 differential染色后，在光学显微镜下检查脱墨报纸纸浆，并在扫描电子显微镜和透射电子显微镜下进行检查，结果显示有原纤化和穿孔现象。
