Enzyme and Fermentation Technology Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak-124001, Haryana, India.
Department of Mechanical Engineering, University Institute of Engineering and Technology (UIET), Maharshi Dayanand University, Rohtak-124001, Haryana, India.
Bioresour Technol. 2024 Sep;408:131173. doi: 10.1016/j.biortech.2024.131173. Epub 2024 Jul 29.
This study reports the cellulo-xylanolytic cocktail production from Hypocrea lixii GGRK4 using multi-objective genetic algorithm-artificial neural network tool, resulting in 8.32 ± 1.07 IU/mL, 51.53 ± 3.78 IU/mL activity of CMCase and xylanase, respectively with more than 85 % residual activity at 60 °C and pH 6.0. Interestingly, metal ions viz. K and Ca stimulated the enzyme activity, whereas Fe and Cu reduced the activity. Significant amounts of hydrophobic compounds, chromophores, and phenolics were released after wastepapers deinking. The deinking efficiency of 73.60 ± 2.45 % and 38.60 ± 1.34 % was obtained for photocopier paper and newspaper, respectively, whereas brightness of 89.90 ± 2.10 % ISO and 44.90 ± 1.63 % ISO was reported for both types of waste papers. The physical strength of deinked photocopier paper and newspapers, i.e., tensile index (3.10 and 0.50 %), tearing index (7.10 and 4.83 %), and burst factor (8.61) were enhanced whereas double fold property was decreased proving wastepaper reusability. This consortium showed effective and significant enzymatic deinking efficiency for recycled wastepapers.
本研究利用多目标遗传算法-人工神经网络工具，从 Hypocrea lixii GGRK4 中生产出纤维素-木聚糖酶混合物，其 CMCase 和木聚糖酶的活性分别为 8.32±1.07IU/mL 和 51.53±3.78IU/mL，在 60°C 和 pH6.0 下具有超过 85%的剩余活性。有趣的是，金属离子 K 和 Ca 能刺激酶的活性，而 Fe 和 Cu 则降低了酶的活性。废纸脱墨后释放出大量的疏水性化合物、发色团和酚类物质。胶印纸和新闻纸的脱墨效率分别为 73.60±2.45%和 38.60±1.34%，而两种废纸的白度分别为 89.90±2.10% ISO 和 44.90±1.63% ISO。脱墨后的胶印纸和新闻纸的物理强度，即抗张指数（3.10 和 0.50%）、撕裂指数（7.10 和 4.83%）和耐破指数（8.61）都有所提高，而双折性能则有所下降，证明了废纸的可重复使用性。该联合体对回收废纸显示出有效的、显著的酶促脱墨效率。
