Clavel Marion, Pélissier Thierry, Montavon Thomas, Tschopp Marie-Aude, Pouch-Pélissier Marie-Noëlle, Descombin Julie, Jean Viviane, Dunoyer Patrice, Bousquet-Antonelli Cécile, Deragon Jean-Marc
UMR5096 LGDP, Université de Perpignan Via Domitia, 58 Avenue Paul Alduy, 66860, Perpignan Cedex, France.
CNRS UMR5096 LGDP, Perpignan Cedex, France.
Plant Mol Biol. 2016 May;91(1-2):131-47. doi: 10.1007/s11103-016-0448-9. Epub 2016 Feb 9.
In this work, we retrace the evolutionary history of plant double-stranded RNA binding proteins (DRBs), a group of non-catalytic factors containing one or more double-stranded RNA binding motif (dsRBM) that play important roles in small RNA biogenesis and functions. Using a phylogenetic approach, we show that multiple dsRBM DRBs are systematically composed of two different types of dsRBMs evolving under different constraints and likely fulfilling complementary functions. In vascular plants, four distinct clades of multiple dsRBM DRBs are always present with the exception of Brassicaceae species, that do not possess member of the newly identified clade we named DRB6. We also identified a second new and highly conserved DRB family (we named DRB7) whose members possess a single dsRBM that shows concerted evolution with the most C-terminal dsRBM domain of the Dicer-like 4 (DCL4) proteins. Using a BiFC approach, we observed that Arabidopsis thaliana DRB7.2 (AtDRB7.2) can directly interact with AtDRB4 but not with AtDCL4 and we provide evidence that both AtDRB7.2 and AtDRB4 participate in the epigenetically activated siRNAs pathway.
在这项研究中，我们追溯了植物双链RNA结合蛋白（DRB）的进化历史，这是一组非催化因子，含有一个或多个双链RNA结合基序（dsRBM），在小RNA的生物合成和功能中发挥重要作用。通过系统发育分析方法，我们发现多个dsRBM DRB由两种不同类型的dsRBM组成，它们在不同的限制条件下进化，可能发挥互补功能。在维管植物中，除十字花科植物外，多个dsRBM DRB总是存在四个不同的进化枝，十字花科植物不具有我们命名为DRB6的新鉴定进化枝的成员。我们还鉴定出了第二个新的高度保守的DRB家族（我们命名为DRB7），其成员具有单个dsRBM，与类Dicer 4（DCL4）蛋白的最C端dsRBM结构域协同进化。使用双分子荧光互补（BiFC）方法，我们观察到拟南芥DRB7.2（AtDRB7.2）可以直接与AtDRB4相互作用，但不能与AtDCL4相互作用，并且我们提供证据表明AtDRB7.2和AtDRB4都参与了表观遗传激活的小干扰RNA（siRNA）途径。
