墨西哥湾沿海沉积物微观世界中细菌群落暴露于马孔多原油（MC252）后的宏基因组数据。
Metagenomic data of the bacterial community in coastal Gulf of Mexico sediment microcosms following exposure to Macondo oil (MC252).
作者信息
Koo Hyunmin, Bej Asim K
机构信息
Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
出版信息
Data Brief. 2015 Nov 26;6:89-93. doi: 10.1016/j.dib.2015.11.040. eCollection 2016 Mar.
Abstract
The data in this article includes the sequences of bacterial 16S rRNA gene from metagenome of Macondo oil (MC252)-treated and non-oil-treated sediment microcosms, collected from coastal Gulf of Mexico and Bayou La Batre, USA. Metacommunity DNA was PCR amplified with 341F and 907R oligonucleotide primers, targeting V3-V5 regions of the 16S rRNA gene. Data were generated by using bacterial tag-encoded FLX-amplicon pyrosequencing (bTEFAP) methodology and then processed using bioinformatics tools such as QIIME. The data information is deposited to NCBI׳s BioProject and BioSample and raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database.
摘要
本文中的数据包括来自美国墨西哥湾沿岸和拉巴特湾经马孔多原油（MC252）处理和未经原油处理的沉积物微观世界宏基因组的细菌16S rRNA基因序列。使用341F和907R寡核苷酸引物对元群落DNA进行PCR扩增，靶向16S rRNA基因的V3-V5区域。数据通过细菌标签编码的FLX扩增子焦磷酸测序（bTEFAP）方法生成，然后使用QIIME等生物信息学工具进行处理。数据信息已存入NCBI的生物项目和生物样本，原始序列文件可通过NCBI的序列读取存档（SRA）数据库获得。
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