Erdogan Innocentia G, Mekuto Lukhanyo, Ntwampe Seteno K O, Fosso-Kankeu Elvis, Waanders Frans B
Water Pollution Monitoring and Remediation Initiatives Research Group in the CoE C-based Fuels School of Chemical and Minerals Engineering, Faculty of Engineering, North-West University, Potchefstroom, South Africa.
Bioresource Engineering Research Group (BioERG), Cape Peninsula University of Technology, Cape Town, South Africa.
Data Brief. 2019 Jun 11;25:104135. doi: 10.1016/j.dib.2019.104135. eCollection 2019 Aug.
The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O'Kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina reaction kits as per manufactures guidelines and sequenced using the illumina MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified.
本文呈现的宏基因组数据包含了南非纳马夸兰奥基普市饮用水供应系统（DWSS）的细菌群落概况。对从水源（奥兰治河）到使用点（奥基普）的代表性样本进行了分析，该样本通过一个用于饮用水分配的150公里长的DWSS，分析其细菌含量。在DNA提取后，使用寡核苷酸引物27F和518R对16S rRNA V1-V3区域进行PCR扩增。PCR扩增产物按照制造商指南使用Illumina反应试剂盒进行处理，并使用Illumina MiSeq-2000通过MiSeq V3试剂盒进行测序。所获得的数据使用生物信息学QIIME软件和兼容的快速核酸（fna）文件进行处理。原始序列存于国家生物技术中心（NCBI）和序列读取存档（SRA）数据库，为每个鉴定出的物种获取了登录号。
