Lasabová Zora, Vazan Martin, Zibolenova Jana, Svecova Iveta
Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia.
Department of Public Health, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia.
Neuro Endocrinol Lett. 2015 Dec;36(7):695-9.
Preeclampsia is a pregnancy-associated disease with the impact of genetic, epigenetic and environmental factors. Increased apoptosis was observed in cells from preeclamptic placentas. MicroRNAs are involved in the regulation of apoptosis and are abundant in placenta. In this study, we focused on the analysis of differential gene expression of apoptosis-associated miRNAs in preeclamptic placenta samples compared to the samples obtained from healthy pregnant women.
MicroRNA was extracted from placental samples of patients with preeclampsia and physiological course of the pregnancy. The gene expression of miR-155, miR-122 and miR-21 in placenta and control samples was estimated by relative quantitation (RQ) using TaqMan probes, normalized against RNU44. The RQ mean values were statistically evaluated by Man-Whitney test.
Using the relative gene expression analysis, we could observe a significant increase in gene expression of miR-155 (p<0.001), miR-21 (p<0.0001) and miR-122 (p<0.01) in preeclamptic placentas.
The apoptosis-associated miRNAs miR-21 and miR-122 are dysregulated in the term preeclamptic placentas. The increased miRNA expression suggest the downregulation of potential targets mRNAs, which can contribute to the pathogenesis of preeclampsia. The identification of their targets in placenta will improve our understanding of their role in preeclampsia.
子痫前期是一种与妊娠相关的疾病，受遗传、表观遗传和环境因素影响。在子痫前期胎盘的细胞中观察到凋亡增加。微小RNA参与细胞凋亡的调控，且在胎盘中含量丰富。在本研究中，我们重点分析了子痫前期胎盘样本与健康孕妇样本相比，凋亡相关微小RNA的差异基因表达。
从子痫前期患者及正常妊娠过程的胎盘样本中提取微小RNA。使用TaqMan探针通过相对定量（RQ）法评估胎盘和对照样本中miR-155、miR-122和miR-21的基因表达，并以RNU44作为标准化对照。RQ平均值通过曼-惠特尼检验进行统计学评估。
通过相对基因表达分析，我们观察到子痫前期胎盘中miR-155（p<0.001）、miR-21（p<0.0001）和miR-122（p<0.01）的基因表达显著增加。
凋亡相关微小RNA miR-21和miR-122在足月子痫前期胎盘中表达失调。微小RNA表达增加表明潜在靶标信使核糖核酸的下调，这可能导致子痫前期的发病机制。确定它们在胎盘中的靶标将增进我们对子痫前期中它们作用的理解。
