[产聚乙烯醇降解酶菌株的分离及发酵条件]

[Isolation and fermentation conditions of polyvinyl alcohol-degrading enzyme producing strain].

作者信息

Xiao C S, Zhang W, Xie Z K, Cao J W, Li X L

出版信息

Wei Sheng Wu Xue Bao. 1989 Oct;29(5):343-7.

Abstract

A bacterium D8 strain which high efficiently degrading PVA was isolated from waste water of factory. The strain possesses the abilities of completely degrading 0.5 per cent of PVA (500, 1700) included in the culture medium for four days. It was identified Pseudomonas pseudoalcaligenes. Fermentation conditions of the strain have been investigated. The suitable medium consisted of PVA 1.5% (NH4)2SO40.1%, K2HPO4 0.24%, KH2PO4 0.04%, MgSO4.7H2O 0.035%, NaCl 0.01%, FeSO4 0.001%, yeast extract 0.15%, pH 7.5. The optimal condition for enzyme production are as follows: 250 ml shake filled with 30 ml medium, 30 degrees C, 160n/min incubation period 72 h. Under such conditions enzyme activity is highest.

摘要

从工厂废水中分离出一株高效降解聚乙烯醇（PVA）的细菌D8菌株。该菌株具有在四天内完全降解培养基中0.5%（500、1700）PVA的能力。经鉴定为假产碱假单胞菌。对该菌株的发酵条件进行了研究。合适的培养基组成为：PVA 1.5%、硫酸铵0.1%、磷酸氢二钾0.24%、磷酸二氢钾0.04%、七水硫酸镁0.035%、氯化钠0.01%、硫酸亚铁0.001%、酵母提取物0.15%，pH值7.5。产酶的最佳条件如下：250毫升摇瓶装30毫升培养基，30℃，160转/分钟，培养72小时。在此条件下酶活性最高。