Kyoto Prefectural University, Shimogamo-nakaragi-cho, Sakyo-ku, Kyoto 606-8522, Japan.
J Biosci Bioeng. 2011 Jul;112(1):58-62. doi: 10.1016/j.jbiosc.2011.03.004. Epub 2011 Apr 17.
To examine the biodegradation of polyvinyl alcohol (PVA), Flammulina velutipes (Enokitake) was cultivated in both liquid and quartz sand cultures. After incubation, discoloration of an iodide solution was observed in the filtrate recovered from the quartz sand culture, whereas discoloration was not observed in the filtrate recovered from the liquid culture. Gel permeation chromatography showed that the PVA recovered from the quartz sand culture was depolymerized and yielded low-molecular-weight portions. Infrared and ultraviolet spectrometry indicated that there was formation of carbonyl groups, and NMR analysis showed that the syndiotactic portions of PVA were preferably attacked. Based on these results, an unsubmerged cultivation of F. velutipes was considered suitable for the biodegradation of PVA, probably because the mycelium was sufficiently developed to produce PVA-degrading enzymes.
为了研究聚乙烯醇（PVA）的生物降解性，将金针菇（滑子菇）分别在液体和石英砂培养中进行培养。培养后，从石英砂培养中回收的滤液中观察到碘化物溶液变色，而从液体培养中回收的滤液中则没有观察到变色。凝胶渗透色谱法表明，从石英砂培养中回收的 PVA 发生了解聚，生成了低分子量部分。红外和紫外光谱表明形成了羰基，NMR 分析表明 PVA 的间同立构部分优先受到攻击。基于这些结果，认为金针菇的未浸没培养适合 PVA 的生物降解，可能是因为菌丝体足够发达，能够产生 PVA 降解酶。
