Muto M G, Lepisto E M, Van den Abbeele A D, Knapp R C, Kassis A I
Department of Obstetrics and Gynecology, Harvard Medical School, Boston, MA.
Am J Obstet Gynecol. 1989 Nov;161(5):1206-12. doi: 10.1016/0002-9378(89)90667-4.
Human antimurine antibody responses interfere with CA 125 antigen determinations by crosslinking the murine antiovarian carcinoma monoclonal antibody OC 125 with the second murine radiolabeled antibody used in the CA 125 radioimmunoassay. Serial CA 125 levels in 22 patients with epithelial ovarian carcinoma undergoing either radioimmunotherapy or radioimmunoscintigraphy with iodine 131-labeled F(ab')2 fragments of OC 125 were followed up for up to 96 weeks after infusion. Fourteen radioimmunoscintigraphy patients received 131I-labeled monoclonal antibody by the intraperitoneal (n = 5) or intravenous (n = 9) route: 10 of 14 had sera drawn at appropriate time points for human antimurine antibody detection; 8 of 10 had 1.3- to 363-fold increases in CA 125; 4 of 8 had detectable human antimurine antibody (18.5 to 22 and 575 to 36 micrograms/ml). Eight radioimmunotherapy patients received 131I-labeled monoclonal antibody by the intraperitoneal route: 8 of 8 displayed an apparent 4.8- to 3725-fold increase in CA 125 levels within 7 to 42 days after monoclonal antibody infusion; 6 of 8 had detectable human antimurine antibody (13 to 4 and 319 to 31 micrograms/ml). Adsorption of immunoglobulin G resulted in a 21% to 98% reduction in CA 125 antigen levels in 4 of 4 patients tested. In patients with demonstrable human antimurine antibody, CA 125 antigen levels obtained by the clinical CA 125 radioimmunoassay are spuriously elevated.
人抗鼠抗体反应通过使鼠抗卵巢癌单克隆抗体OC 125与CA 125放射免疫分析中使用的第二种鼠放射性标记抗体交联，干扰CA 125抗原的测定。对22例接受131碘标记的OC 125 F(ab')2片段进行放射免疫治疗或放射免疫闪烁成像的上皮性卵巢癌患者，在输注后长达96周内随访其CA 125水平。14例放射免疫闪烁成像患者通过腹腔内（n = 5）或静脉内（n = 9）途径接受131I标记的单克隆抗体：14例中有10例在适当时间点采集血清检测人抗鼠抗体；10例中有8例CA 125升高1.3至363倍；8例中有4例可检测到人抗鼠抗体（18.5至22和575至36微克/毫升）。8例放射免疫治疗患者通过腹腔内途径接受131I标记的单克隆抗体：8例中有8例在单克隆抗体输注后7至42天内CA 125水平明显升高4.8至3725倍；8例中有6例可检测到人抗鼠抗体（13至4和319至31微克/毫升）。在4例检测的患者中，免疫球蛋白G的吸附导致CA 125抗原水平降低21%至98%。在可检测到人抗鼠抗体的患者中，临床CA 125放射免疫分析获得的CA 125抗原水平会假性升高。
