Reinsberg J, Wagner U, Krebs D
Zentrum für Frauenheilkunde und Geburtshilfe, Universität Bonn, Germany.
Arch Gynecol Obstet. 1994;255(1):9-18. doi: 10.1007/BF02390669.
The influence of human anti-OC125 antibodies formed after multiple infusions of OC125 F(ab')2 fragments on the apparent levels of CA 125 measured with four different tests were examined in two ovarian cancer patients. With the homologous Assay 1, involving only OC125 antibodies, false increases of CA 125 values were observed after infusion of OC125 fragments, which completely covered real CA 125. In contrast, with Assay 2, 3 and 4, which involve no OC125 antibodies as capture antibodies, only slight false increases occurred in the presence of very high anti-OC125 antibody concentrations. Interference was eliminated by addition of non-specific murine IgG in Assay 2 and 4, but not in Assay 1 and 3 indicating that the false increases in Assay 1 and 3 were caused by anti-idiotypic anti-OC125 antibodies. In the presence of elevated real CA 125, with Assay 2 and to a considerably lesser degree with Assay 4, an inhibitory effect of anti-OC125 antibodies became evident leading to false decreases of CA 125 values. In Assay 4 reduction of assay response was eliminated by addition of nonspecific murine IgG. The results confirm that all available CA 125 tests are influenced by interference with human anti-OC125 antibodies. Thus, CA 125 levels in patients who have been treated with OC125 fragments should be interpreted with care.
在两名卵巢癌患者中，研究了多次输注OC125 F(ab')2片段后形成的人抗OC125抗体对用四种不同检测方法测得的CA 125表观水平的影响。使用仅涉及OC125抗体的同源检测方法1，输注OC125片段后观察到CA 125值出现假升高，这完全掩盖了实际的CA 125。相比之下，使用不涉及OC125抗体作为捕获抗体的检测方法2、3和4，在抗OC125抗体浓度非常高的情况下仅出现轻微的假升高。在检测方法2和4中，通过添加非特异性鼠IgG消除了干扰，但在检测方法1和3中未消除，这表明检测方法1和3中的假升高是由抗独特型抗OC125抗体引起的。在实际CA 125升高的情况下，在检测方法2中以及在检测方法4中程度要小得多，抗OC125抗体的抑制作用变得明显，导致CA 125值出现假降低。在检测方法4中，通过添加非特异性鼠IgG消除了检测反应的降低。结果证实，所有可用的CA 125检测方法都会受到人抗OC125抗体干扰的影响。因此，对于接受过OC125片段治疗的患者，CA 125水平的解读应谨慎。
