Ohashi Rie, Takao Keizo, Miyakawa Tsuyoshi, Shiina Nobuyuki
Laboratory of Neuronal Cell Biology, National Institute for Basic Biology, Okazaki, Aichi, Japan.
Department of Basic Biology, SOKENDAI, Okazaki, Aichi, Japan.
Sci Rep. 2016 Feb 11;6:20775. doi: 10.1038/srep20775.
RNG105 (also known as Caprin1) is a major RNA-binding protein in neuronal RNA granules, and is responsible for mRNA transport to dendrites and neuronal network formation. A recent study reported that a heterozygous mutation in the Rng105 gene was found in an autism spectrum disorder (ASD) patient, but it remains unclear whether there is a causal relation between RNG105 deficiency and ASD. Here, we subjected Rng105(+/-) mice to a comprehensive behavioral test battery, and revealed the influence of RNG105 deficiency on mouse behavior. Rng105(+/-) mice exhibited a reduced sociality in a home cage and a weak preference for social novelty. Consistently, the Rng105(+/-) mice also showed a weak preference for novel objects and novel place patterns. Furthermore, although the Rng105(+/-) mice exhibited normal memory acquisition, they tended to have relative difficulty in reversal learning in the spatial reference tasks. These findings suggest that the RNG105 heterozygous knockout leads to a reduction in sociality, response to novelty and flexibility in learning, which are implicated in ASD-like behavior.
RNG105（也称为Caprin1）是神经元RNA颗粒中的一种主要RNA结合蛋白，负责将mRNA转运至树突并形成神经网络。最近一项研究报告称，在一名自闭症谱系障碍（ASD）患者中发现了Rng105基因的杂合突变，但RNG105缺陷与ASD之间是否存在因果关系仍不清楚。在此，我们对Rng105(+/-)小鼠进行了全面的行为测试组，并揭示了RNG105缺陷对小鼠行为的影响。Rng105(+/-)小鼠在笼内表现出社交性降低，对社交新奇性的偏好较弱。同样，Rng105(+/-)小鼠对新物体和新位置模式也表现出较弱的偏好。此外，尽管Rng105(+/-)小鼠表现出正常的记忆获取能力，但它们在空间参考任务的逆向学习中往往相对困难。这些发现表明，RNG105杂合敲除导致社交性降低、对新奇事物的反应以及学习灵活性下降，这些都与ASD样行为有关。
