孕期母鼠食用富含ω-6脂肪酸且ω-3脂肪酸含量低的饮食所生的小鼠表现出各种行为变化，包括社交行为受损和识别记忆增强。

Mice Born to Mothers Fed a Diet High in Omega-6 Fatty Acids and Low in Omega-3 Fatty Acids During Pregnancy Exhibit Various Behavioral Changes Including Impaired Social Behaviors and Enhanced Recognition Memory.

作者信息

Sakayori Nobuyuki, Fujii Kazuki, Katakura Masanori, Adachi Mayumi, Koshidaka Yumie, Takao Keizo, Sugita Makoto

机构信息

Department of Physiology and Oral Physiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Department of Behavioral Physiology, Faculty of Medicine, University of Toyama, Toyama, Japan; Life Science Research Center, University of Toyama, Toyama, Japan; Research Center for Idling Brain Science, University of Toyama, Toyama, Japan.

出版信息

J Nutr. 2025 Mar;155(3):775-787. doi: 10.1016/j.tjnut.2024.12.031. Epub 2025 Jan 3.

DOI:10.1016/j.tjnut.2024.12.031

PMID:39755239

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11934248/

Abstract

BACKGROUND

Modern dietary trends have led to an increase in foods that are relatively high in n-6 PUFAs and low in n-3 PUFAs. We previously reported that the offspring of mother mice that consumed a diet high in n-6 linoleic acid (LA) and low in n-3 α-linolenic acid (ALA), hereinafter called the LA/ALA diet, exhibited behavioral abnormalities related to anxiety and feeding.

OBJECTIVES

We currently lack a comprehensive overview of the behavioral abnormalities in these offspring, which was investigated in this study.

METHODS

C57BL/6J virgin female mice at 11 wk of age were fed either a control diet or the LA/ALA diet, mated at 13 wk of age, and maintained on their respective diet throughout gestation. At birth, the lactating mothers' diet was replaced with standard laboratory feed pellets. After weaning, the offspring continued to receive standard laboratory feed pellets, and both male and female offspring at 1-63 wk of age were analyzed using a comprehensive behavioral test battery (n = 6-14 offspring/group and offspring in each group were derived from ≥3 independent litters).

RESULTS

Both male and female offspring exposed in utero to the LA/ALA diet exhibited impaired social behaviors, including the lower number of contacts with novel mice in the social interaction test [diet, F = 9.807, P = 0.007, 2-way analysis of variance (ANOVA)], and also showed enhanced recognition memory in the object location test (diet, F = 6.779, P = 0.013, 2-way ANOVA) compared with offspring exposed in utero to the control diet. In addition, compared with sex-matched controls, female offspring displayed hyperactivity in the open field test (F = 6.097, P = 0.018, simple main effect analysis).

CONCLUSIONS

The maternal balance between dietary n-6 and n-3 PUFAs during pregnancy can have significant effects on the offspring's behaviors, lasting well into adulthood.

摘要

背景

现代饮食趋势导致富含n-6多不饱和脂肪酸（PUFAs）且n-3 PUFAs含量低的食物摄入增加。我们之前报道过，食用富含n-6亚油酸（LA）且n-3α-亚麻酸（ALA）含量低的饮食（以下简称LA/ALA饮食）的母鼠后代表现出与焦虑和进食相关的行为异常。

目的

本研究对这些后代的行为异常进行了调查，目前我们缺乏对其行为异常的全面概述。

方法

11周龄的C57BL/6J处女雌性小鼠分别喂食对照饮食或LA/ALA饮食，13周龄时交配，并在整个妊娠期维持各自的饮食。出生时，将哺乳期母亲的饮食换成标准实验室饲料颗粒。断奶后，后代继续接受标准实验室饲料颗粒，对1至63周龄的雄性和雌性后代使用综合行为测试组进行分析（每组n = 6 - 14只后代，每组后代来自≥3个独立窝）。

结果

在子宫内接触LA/ALA饮食的雄性和雌性后代均表现出社交行为受损，包括在社交互动测试中与陌生小鼠接触次数减少[饮食，F = 9.807，P = 0.007，双向方差分析（ANOVA）]，并且与在子宫内接触对照饮食的后代相比，在物体位置测试中表现出增强的识别记忆（饮食，F = 6.779，P = 0.013，双向ANOVA）。此外，与性别匹配的对照组相比，雌性后代在旷场测试中表现出多动（F = 6.097，P = 0.018，简单主效应分析）。