Baruah P, Deka S, Baruah P P
Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science & Technology (IASST), Paschim Boragaon, Guwahati, 781 035, Assam, India.
Department of Botany, Gauhati University, Guwahati, 781014, Assam, India.
Environ Sci Pollut Res Int. 2016 Jun;23(11):10595-10603. doi: 10.1007/s11356-016-6227-y. Epub 2016 Feb 11.
The purpose of the study was to evaluate the phytoremediation potentiality of a herb named Crotalaria pallida which are abundantly grown on crude oil-contaminated soil of oil field situated at upper Assam, India, so that this plant could be used to remediate hydrocarbon from contaminated soil. To evaluate the potentiality of the plant, a pot culture experiment was conducted taking 3 kg of rice field soil mixed with crude oil at a concentration of 10,000 (10 g/kg), 20,000 (20 g/kg), 30,000 (30 g/kg), 40,000 (40 g/kg), 50,000 (50 g/kg), 60,000 (60 g/kg), 70,000 (70 g/kg), 80,000 (80 g/kg), 90,000 (90 g/kg), and 100,000 (100 g/kg) ppm. Ten numbers of healthy seeds of C. pallida were sown in three pots of each concentration for germination, and after 15 days of germination, single healthy seedling in each pot was kept for the study. A control setup was also maintained without adding crude oil. The duration of the experiment was fixed for 6 months. The results showed that uptake of hydrocarbon by the plants was increased with increasing the concentration of crude oil in the soil up to 60,000 ppm. After that, uptake of hydrocarbon by the plants was found to be lower with increasing doses of crude oil concentration. Uptake of hydrocarbon by the shoot was found to be maximum, i.e., 35,018 ppm in 60,000 ppm concentration. Dissipation of total petroleum hydrocarbon (TPH) from the soil was also gradually increased with increasing concentration of crude oil in the soil up to 60,000 ppm. Maximum dissipation, i.e., 78.66 %, occurred in 60,000 ppm concentration of crude oil-mixed soil. The plant could not survive in 100,000 ppm concentration of crude oil-mixed soil. The results also demonstrated that there was a reduction in plant shoot and root biomass with an increase of crude oil concentration. Furthermore, results revealed that the shoot biomass was higher than root biomass in all the treatments.
该研究的目的是评估一种名为猪屎豆的草本植物的植物修复潜力，这种植物在印度阿萨姆邦上游油田受原油污染的土壤上大量生长，以便该植物可用于修复受污染土壤中的碳氢化合物。为了评估该植物的潜力，进行了盆栽试验，取3千克稻田土壤，分别与浓度为10000（10克/千克）、20000（20克/千克）、30000（30克/千克）、40000（40克/千克）、50000（50克/千克）、60000（60克/千克）、70000（70克/千克）、80000（80克/千克）、90000（90克/千克）和100000（100克/千克）ppm的原油混合。在每个浓度的三个花盆中播种十粒健康的猪屎豆种子用于发芽，发芽15天后，每个花盆中保留一株健康的幼苗用于研究。还设置了不添加原油的对照装置。实验持续时间定为6个月。结果表明，在土壤中原油浓度增加到60000 ppm之前，植物对碳氢化合物的吸收量随原油浓度的增加而增加。在此之后，随着原油浓度剂量的增加，植物对碳氢化合物的吸收量较低。地上部分对碳氢化合物的吸收量最大，即在60000 ppm浓度下为35018 ppm。土壤中总石油烃（TPH）的消散也随着土壤中原油浓度的增加而逐渐增加，直至60000 ppm。在60000 ppm浓度的原油混合土壤中，最大消散率为78.66%。该植物在100000 ppm浓度的原油混合土壤中无法存活。结果还表明，随着原油浓度的增加，植物地上部分和根部生物量减少。此外，结果显示在所有处理中地上部分生物量高于根部生物量。
